Evolutionary dynamics of a conserved sequence motif in the ribosomal genes of the ciliate Paramecium

Francesco Catania, Michael Lynch

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. In protozoa, the identification of preserved motifs by comparative genomics is often impeded by difficulties to generate reliable alignments for non-coding sequences. Moreover, the evolutionary dynamics of regulatory elements in 3' untranslated regions (both in protozoa and metazoa) remains a virtually unexplored issue. Results. By screening Paramecium tetraurelia's 3' untranslated regions for 8-mers that were previously found to be preserved in mammalian 3' UTRs, we detect and characterize a motif that is distinctly conserved in the ribosomal genes of this ciliate. The motif appears to be conserved across Paramecium aurelia species but is absent from the ribosomal genes of four additional non-Paramecium species surveyed, including another ciliate, Tetrahymena thermophila. Motif-free ribosomal genes retain fewer paralogs in the genome and appear to be lost more rapidly relative to motif-containing genes. Features associated with the discovered preserved motif are consistent with this 8-mer playing a role in post-transcriptional regulation. Conclusions. Our observations 1) shed light on the evolution of a putative regulatory motif across large phylogenetic distances; 2) are expected to facilitate the understanding of the modulation of ribosomal genes expression in Paramecium; and 3) reveal a largely unexplored - and presumably not restricted to Paramecium - association between the presence/absence of a DNA motif and the evolutionary fate of its host genes.

Original languageEnglish (US)
Article number129
JournalBMC Evolutionary Biology
Volume10
Issue number1
DOIs
StatePublished - Jun 2 2010
Externally publishedYes

Fingerprint

Paramecium
conserved sequences
ciliate
Ciliophora
3' untranslated regions
gene
genes
Protozoa
Paramecium tetraurelia
Tetrahymena thermophila
Animalia
gene expression
genomics
genome
screening
phylogenetics
DNA
phylogeny

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Evolutionary dynamics of a conserved sequence motif in the ribosomal genes of the ciliate Paramecium. / Catania, Francesco; Lynch, Michael.

In: BMC Evolutionary Biology, Vol. 10, No. 1, 129, 02.06.2010.

Research output: Contribution to journalArticle

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