TY - JOUR
T1 - Evolutionary conservation of a core fungal phosphate homeostasis pathway coupled to development in Blastocladiella emersonii
AU - Gomes-Vieira, André L.
AU - Wideman, Jeremy G.
AU - Paes-Vieira, Lisvane
AU - Gomes, Suely L.
AU - Richards, Thomas A.
AU - Meyer-Fernandes, José Roberto
N1 - Funding Information:
We would like to thank Fabiano Ferreira Esteves, Edimilson Antonio Pereira and Rosangela Rosa de Araújo for their excellent technical assistance. This work was supported by grants from the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq – Grant Number: 401134/2014–8 ), Coordenação de Aperfeiçoamento de Pessoal de Nível superior (CAPES – Grant Number: 0012017 ) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ – Grant Number: e-26/ 201.300/2014 ) to JRM-F, ALG-V and LP-V. SLG is partially supported by CNPq. TAR is supported by a Royal Society University Research Fellowship with additional grant support from the Leverhulme Trust , Biotechnology and Biological Sciences Research Council (BBSRC – Grant Number: BB/G00885X/1 ), European Molecular Biology Organization (EMBO), Canadian Institute for Advanced Research (CIFAR), Natural Environment Research Council (NERC), and the Gordon and Betty Moore Foundation . JGW is supported by the European Molecular Biology Organization Long-term Fellowship ( ALTF 761-2014 ) co-funded by European Commission ( EMBOCOFUND2012 , GA-2012-600394 ) support from Marie Curie Actions.
Funding Information:
We would like to thank Fabiano Ferreira Esteves, Edimilson Antonio Pereira and Rosangela Rosa de Araújo for their excellent technical assistance. This work was supported by grants from the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq – Grant Number: 401134/2014–8), Coordenação de Aperfeiçoamento de Pessoal de Nível superior (CAPES – Grant Number: 0012017) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ – Grant Number: e-26/ 201.300/2014) to JRM-F, ALG-V and LP-V. SLG is partially supported by CNPq. TAR is supported by a Royal Society University Research Fellowship with additional grant support from the Leverhulme Trust, Biotechnology and Biological Sciences Research Council (BBSRC – Grant Number: BB/G00885X/1), European Molecular Biology Organization (EMBO), Canadian Institute for Advanced Research (CIFAR), Natural Environment Research Council (NERC), and the Gordon and Betty Moore Foundation. JGW is supported by the European Molecular Biology Organization Long-term Fellowship (ALTF 761-2014) co-funded by European Commission (EMBOCOFUND2012, GA-2012-600394) support from Marie Curie Actions.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/6
Y1 - 2018/6
N2 - The model yeast Saccharomyces cerevisiae elicits a transcriptional response to phosphate (P i ) depletion. To determine the origins of the phosphate response (PHO) system, we bioinformatically identified putative PHO components in the predicted proteomes of diverse fungi. Our results suggest that the PHO system is ancient; however, components have been expanded or lost in different fungal lineages. To show that a similar physiological response is present in deeply-diverging fungi we examined the transcriptional and physiological response of PHO genes to P i depletion in the blastocladiomycete Blastocladiella emersonii. Our physiological experiments indicate that B. emersonii relies solely on high-affinity Na + -independent Pho84-like transporters. In response to P i depletion, BePho84 paralogues were 4–8-fold transcriptionally upregulated, whereas several other PHO homologues like phosphatases and vacuolar transporter chaperone (VTC) complex components show 2–3-fold transcriptional upregulation. Since P i has been shown to be important during the development of B. emersonii, we sought to determine if PHO genes are differentially regulated at different lifecycle stages. We demonstrate that a similar set of PHO transporters and phosphatases are upregulated at key points during B. emersonii development. Surprisingly, some genes upregulated during P i depletion, including VTC components, are repressed at these key stages of development indicating that PHO genes are regulated by different pathways in different developmental and environmental situations. Overall, our findings indicate that a complex PHO network existed in the ancient branches of the fungi, persists in diverse extant fungi, and that this ancient network is likely to be involved in development and cell cycle regulation.
AB - The model yeast Saccharomyces cerevisiae elicits a transcriptional response to phosphate (P i ) depletion. To determine the origins of the phosphate response (PHO) system, we bioinformatically identified putative PHO components in the predicted proteomes of diverse fungi. Our results suggest that the PHO system is ancient; however, components have been expanded or lost in different fungal lineages. To show that a similar physiological response is present in deeply-diverging fungi we examined the transcriptional and physiological response of PHO genes to P i depletion in the blastocladiomycete Blastocladiella emersonii. Our physiological experiments indicate that B. emersonii relies solely on high-affinity Na + -independent Pho84-like transporters. In response to P i depletion, BePho84 paralogues were 4–8-fold transcriptionally upregulated, whereas several other PHO homologues like phosphatases and vacuolar transporter chaperone (VTC) complex components show 2–3-fold transcriptional upregulation. Since P i has been shown to be important during the development of B. emersonii, we sought to determine if PHO genes are differentially regulated at different lifecycle stages. We demonstrate that a similar set of PHO transporters and phosphatases are upregulated at key points during B. emersonii development. Surprisingly, some genes upregulated during P i depletion, including VTC components, are repressed at these key stages of development indicating that PHO genes are regulated by different pathways in different developmental and environmental situations. Overall, our findings indicate that a complex PHO network existed in the ancient branches of the fungi, persists in diverse extant fungi, and that this ancient network is likely to be involved in development and cell cycle regulation.
KW - Blastocladiella emersonii
KW - Fungi
KW - PHO pathway
KW - Phosphate
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U2 - 10.1016/j.fgb.2018.04.004
DO - 10.1016/j.fgb.2018.04.004
M3 - Article
C2 - 29627365
AN - SCOPUS:85045683751
SN - 1087-1845
VL - 115
SP - 20
EP - 32
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
ER -