Evolutionary Conservation and Molecular Cloning of the Recombinase Activating Gene 1

R. M. Bernstein, S. F. Schluter, Douglas Lake, J. J. Marchalonis

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A 700-bp fragment of the recombinase activating gene 1 (RAG-1) was cloned from several evolutionarily distant (sandbar shark, paddlefish, goldfish, axolotl and pig) species using PCR. The nucleotide and deduced amino acid sequences revealed a highly conserved region that has remained essentially unaltered during 400 million years of evolution; e.g, shark and human sequences were 75% identical at the nucleic acid level and 87% as protein. The RAG-1 mRNA levels in the shark were analyzed using semi-quantitative PCR to reveal expression patterns contrary to normal mammalian expression. These results establish that the genetic mechisms for Ig gene rearrangement are present in all extant gnathanstomes.

Original languageEnglish (US)
Pages (from-to)687-692
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume205
Issue number1
DOIs
StatePublished - Nov 30 1994
Externally publishedYes

Fingerprint

Sharks
Recombinases
Cloning
Molecular Cloning
Conservation
Genes
Ambystoma mexicanum
Polymerase Chain Reaction
Immunoglobulin Genes
Goldfish
Gene Rearrangement
Nucleic Acids
Amino Acid Sequence
Swine
Nucleotides
Amino Acids
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Evolutionary Conservation and Molecular Cloning of the Recombinase Activating Gene 1. / Bernstein, R. M.; Schluter, S. F.; Lake, Douglas; Marchalonis, J. J.

In: Biochemical and Biophysical Research Communications, Vol. 205, No. 1, 30.11.1994, p. 687-692.

Research output: Contribution to journalArticle

Bernstein, R. M. ; Schluter, S. F. ; Lake, Douglas ; Marchalonis, J. J. / Evolutionary Conservation and Molecular Cloning of the Recombinase Activating Gene 1. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 205, No. 1. pp. 687-692.
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