Evolution of Conformational Dynamics Determines the Conversion of a Promiscuous Generalist into a Specialist Enzyme

Taisong Zou, Valeria A. Risso, Jose A. Gavira, Jose M. Sanchez-Ruiz, Sefika Ozkan

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

β-lactamases are produced by many modern bacteria as a mechanism of resistance toward β-lactam antibiotics, the most common antibiotics in use. β-lactamases, however, are ancient enzymes that originated billions of years ago. Recently, proteins corresponding to 2- to 3-Gy-old Precambrian nodes in the evolution of Class A β-lactamases have been prepared and shown to be moderately efficient promiscuous catalysts, able to degrade a variety of antibiotics with catalytic efficiency levels similar to those of an average modern enzyme. Remarkably, there are few structural differences (in particular at the active-site regions) between the resurrected enzymes and a penicillin-specialist modern β-lactamase. Here, we propose that the ancestral promiscuity originates from conformational dynamics. We investigate the differences in conformational dynamics of the ancient and extant β-lactamases through MD simulations and quantify the contribution of each position to functionally related dynamics through Dynamic Flexibility Index. The modern TEM-1 lactamase shows a comparatively rigid active-site region, likely reflecting adaptation for efficient degradation of a specific substrate (penicillin), whereas enhanced deformability at the active-site neighborhood in the ancestral resurrected proteins likely accounts for the binding and subsequent degradation of antibiotic molecules of different size and shape. Clustering of the conformational dynamics on the basis of Principal Component Analysis is in agreement with the functional divergence, as the ancient β-lactamases cluster together, separated from their modern descendant. Finally, our analysis leads to testable predictions, as sites of potential relevance for the evolution of dynamics are identified and mutations at those sites are expected to alter substrate-specificity.

Original languageEnglish (US)
Pages (from-to)132-143
Number of pages12
JournalMolecular Biology and Evolution
Volume32
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

generalist
antibiotics
enzyme
Anti-Bacterial Agents
Catalytic Domain
Enzymes
enzymes
active sites
Penicillins
penicillins
substrate
Lactams
degradation
protein
Substrate Specificity
Principal Component Analysis
lactams
Cluster Analysis
transmission electron microscopy
mutation

Keywords

  • Ancestral enzyme
  • Molecular dynamics
  • Protein dynamics and structure

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Evolution of Conformational Dynamics Determines the Conversion of a Promiscuous Generalist into a Specialist Enzyme. / Zou, Taisong; Risso, Valeria A.; Gavira, Jose A.; Sanchez-Ruiz, Jose M.; Ozkan, Sefika.

In: Molecular Biology and Evolution, Vol. 32, No. 1, 01.01.2015, p. 132-143.

Research output: Contribution to journalArticle

Zou, Taisong ; Risso, Valeria A. ; Gavira, Jose A. ; Sanchez-Ruiz, Jose M. ; Ozkan, Sefika. / Evolution of Conformational Dynamics Determines the Conversion of a Promiscuous Generalist into a Specialist Enzyme. In: Molecular Biology and Evolution. 2015 ; Vol. 32, No. 1. pp. 132-143.
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