Evolution of Barrett's esophagus through space and time at single-crypt and whole-biopsy levels

Pierre Martinez, Diego Mallo, Thomas G. Paulson, Xiaohong Li, Carissa A. Sanchez, Brian J. Reid, Trevor A. Graham, Mary K. Kuhner, Carlo Maley

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The low risk of progression of Barrett's esophagus (BE) to esophageal adenocarcinoma can lead to over-diagnosis and over-treatment of BE patients. This may be addressed through a better understanding of the dynamics surrounding BE malignant progression. Although genetic diversity has been characterized as a marker of malignant development, it is still unclear how BE arises and develops. Here we uncover the evolutionary dynamics of BE at crypt and biopsy levels in eight individuals, including four patients that experienced malignant progression. We assay eight individual crypts and the remaining epithelium by SNP array for each of 6-11 biopsies over 2 time points per patient (358 samples in total). Our results indicate that most Barrett's segments are clonal, with similar number and inferred rates of alterations observed for crypts and biopsies. Divergence correlates with geographical location, being higher near the gastro-esophageal junction. Relaxed clock analyses show that genomic instability precedes and is enhanced by genome doubling. These results shed light on the clinically relevant evolutionary dynamics of BE.

Original languageEnglish (US)
Article number794
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

esophagus
Barrett Esophagus
Biopsy
progressions
Clocks
Assays
Genes
biological diversity
epithelium
genome
Genomic Instability
markers
clocks
Single Nucleotide Polymorphism
divergence
Adenocarcinoma
Epithelium
Genome

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Evolution of Barrett's esophagus through space and time at single-crypt and whole-biopsy levels. / Martinez, Pierre; Mallo, Diego; Paulson, Thomas G.; Li, Xiaohong; Sanchez, Carissa A.; Reid, Brian J.; Graham, Trevor A.; Kuhner, Mary K.; Maley, Carlo.

In: Nature Communications, Vol. 9, No. 1, 794, 01.12.2018.

Research output: Contribution to journalArticle

Martinez, P, Mallo, D, Paulson, TG, Li, X, Sanchez, CA, Reid, BJ, Graham, TA, Kuhner, MK & Maley, C 2018, 'Evolution of Barrett's esophagus through space and time at single-crypt and whole-biopsy levels' Nature Communications, vol. 9, no. 1, 794. https://doi.org/10.1038/s41467-017-02621-x
Martinez, Pierre ; Mallo, Diego ; Paulson, Thomas G. ; Li, Xiaohong ; Sanchez, Carissa A. ; Reid, Brian J. ; Graham, Trevor A. ; Kuhner, Mary K. ; Maley, Carlo. / Evolution of Barrett's esophagus through space and time at single-crypt and whole-biopsy levels. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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