Evidence of inter-component recombination, intra-component recombination and reassortment in banana bunchy top virus

Daisy Stainton, Simona Kraberger, Matthew Walters, Elizabeth J. Wiltshire, Karyna Rosario, Manáia Halafihi, Samiuela Lolohea, Ika Katoa, Túamelie H. Faitua, Waikato Aholelei, Luseane Taufa, John E. Thomas, David A. Collings, Darren P. Martin, Arvind Varsani

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Banana bunchy top virus (BBTV; family Nanoviridae, genus Babuvirus) is a multi-component, ssDNA virus, which causes widespread banana crop losses throughout tropical Africa and Australasia. We determined the full genome sequences of 12 BBTV isolates from the Kingdom of Tonga and analysed these together with previously determined BBTV sequences to show that reassortment and both inter- and intra-component recombination have all been relatively requent occurrences during BBTV evolution. We found that whereas DNA-U3 components display evidence of complex inter- and intra-component recombination, all of the South Pacific DNA-R components have a common intra-component recombinant origin spanning the replicationassociated protein gene. Altogether, the DNA-U3 and DNA-M components display a greater degree of inter-component recombination than the DNA-R, -S, -C and -M components. The breakpoint distribution of the inter-component recombination events reveals a primary recombination hotspot around the 59 side of the common region major and, in accordance with recombination hotspots detectable in related ssDNA viruses, a secondary recombination hotspot near the origin of virion-strand replication.

Original languageEnglish (US)
Pages (from-to)1103-1119
Number of pages17
JournalJournal of General Virology
Volume93
Issue number5
DOIs
StatePublished - 2012
Externally publishedYes

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Babuvirus
Genetic Recombination
DNA
Myeloma Proteins
Nanoviridae
Tonga
Australasia
Viral Structures
Musa
Virion
Genome
Viruses

ASJC Scopus subject areas

  • Virology

Cite this

Evidence of inter-component recombination, intra-component recombination and reassortment in banana bunchy top virus. / Stainton, Daisy; Kraberger, Simona; Walters, Matthew; Wiltshire, Elizabeth J.; Rosario, Karyna; Halafihi, Manáia; Lolohea, Samiuela; Katoa, Ika; Faitua, Túamelie H.; Aholelei, Waikato; Taufa, Luseane; Thomas, John E.; Collings, David A.; Martin, Darren P.; Varsani, Arvind.

In: Journal of General Virology, Vol. 93, No. 5, 2012, p. 1103-1119.

Research output: Contribution to journalArticle

Stainton, D, Kraberger, S, Walters, M, Wiltshire, EJ, Rosario, K, Halafihi, M, Lolohea, S, Katoa, I, Faitua, TH, Aholelei, W, Taufa, L, Thomas, JE, Collings, DA, Martin, DP & Varsani, A 2012, 'Evidence of inter-component recombination, intra-component recombination and reassortment in banana bunchy top virus', Journal of General Virology, vol. 93, no. 5, pp. 1103-1119. https://doi.org/10.1099/vir.0.040337-0
Stainton, Daisy ; Kraberger, Simona ; Walters, Matthew ; Wiltshire, Elizabeth J. ; Rosario, Karyna ; Halafihi, Manáia ; Lolohea, Samiuela ; Katoa, Ika ; Faitua, Túamelie H. ; Aholelei, Waikato ; Taufa, Luseane ; Thomas, John E. ; Collings, David A. ; Martin, Darren P. ; Varsani, Arvind. / Evidence of inter-component recombination, intra-component recombination and reassortment in banana bunchy top virus. In: Journal of General Virology. 2012 ; Vol. 93, No. 5. pp. 1103-1119.
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