Evidence for preferential breakage of the minus strand of ΦX174 replicative form DNA by a T4-induced endonuclease

Sidney Altman, D. T. Denhardt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

1. 1. A comparison of the actions of pancreatic deoxyribonuclease and a partially purified T4-induced endonuclease on the superhelical F{cyrillic}X174 replicative form (RF I) is presented. Pancreatic deoxyribonuclease appears to introduce single-stranded breaks into RF I without discriminating between the two strands, whereas the T4-induced endonuclease shows a pronounced preference for the "minus" strand. 2. 2. The viral or "plus" strand of RF I is normally about two and a half times as infective in spheroplasts as the complementary "minus" strand.

Original languageEnglish (US)
Pages (from-to)21-28
Number of pages8
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume224
Issue number1
DOIs
StatePublished - Nov 12 1970
Externally publishedYes

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Deoxyribonucleases
Endonucleases
Spheroplasts
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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T1 - Evidence for preferential breakage of the minus strand of ΦX174 replicative form DNA by a T4-induced endonuclease

AU - Altman, Sidney

AU - Denhardt, D. T.

PY - 1970/11/12

Y1 - 1970/11/12

N2 - 1. 1. A comparison of the actions of pancreatic deoxyribonuclease and a partially purified T4-induced endonuclease on the superhelical F{cyrillic}X174 replicative form (RF I) is presented. Pancreatic deoxyribonuclease appears to introduce single-stranded breaks into RF I without discriminating between the two strands, whereas the T4-induced endonuclease shows a pronounced preference for the "minus" strand. 2. 2. The viral or "plus" strand of RF I is normally about two and a half times as infective in spheroplasts as the complementary "minus" strand.

AB - 1. 1. A comparison of the actions of pancreatic deoxyribonuclease and a partially purified T4-induced endonuclease on the superhelical F{cyrillic}X174 replicative form (RF I) is presented. Pancreatic deoxyribonuclease appears to introduce single-stranded breaks into RF I without discriminating between the two strands, whereas the T4-induced endonuclease shows a pronounced preference for the "minus" strand. 2. 2. The viral or "plus" strand of RF I is normally about two and a half times as infective in spheroplasts as the complementary "minus" strand.

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JF - BBA Section Nucleic Acids And Protein Synthesis

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