Evidence for preferential breakage of the minus strand of ΦX174 replicative form DNA by a T4-induced endonuclease

S. Altman; D. T. Denhardt

doi:10.1016/0005-2787(70)90616-7

Evidence for preferential breakage of the minus strand of ΦX174 replicative form DNA by a T4-induced endonuclease

S. Altman, D. T. Denhardt

Research output: Contribution to journal › Article › peer-review

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Abstract

1. 1. A comparison of the actions of pancreatic deoxyribonuclease and a partially purified T4-induced endonuclease on the superhelical F{cyrillic}X174 replicative form (RF I) is presented. Pancreatic deoxyribonuclease appears to introduce single-stranded breaks into RF I without discriminating between the two strands, whereas the T4-induced endonuclease shows a pronounced preference for the "minus" strand. 2. 2. The viral or "plus" strand of RF I is normally about two and a half times as infective in spheroplasts as the complementary "minus" strand.

Original language	English (US)
Pages (from-to)	21-28
Number of pages	8
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	224
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(70)90616-7
State	Published - Nov 12 1970
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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10.1016/0005-2787(70)90616-7

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title = "Evidence for preferential breakage of the minus strand of ΦX174 replicative form DNA by a T4-induced endonuclease",

abstract = "1. 1. A comparison of the actions of pancreatic deoxyribonuclease and a partially purified T4-induced endonuclease on the superhelical F{cyrillic}X174 replicative form (RF I) is presented. Pancreatic deoxyribonuclease appears to introduce single-stranded breaks into RF I without discriminating between the two strands, whereas the T4-induced endonuclease shows a pronounced preference for the {"}minus{"} strand. 2. 2. The viral or {"}plus{"} strand of RF I is normally about two and a half times as infective in spheroplasts as the complementary {"}minus{"} strand.",

author = "S. Altman and Denhardt, {D. T.}",

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T1 - Evidence for preferential breakage of the minus strand of ΦX174 replicative form DNA by a T4-induced endonuclease

AU - Altman, S.

AU - Denhardt, D. T.

N1 - Funding Information: This researchw as supportedb y grants from the United StatesP ublic Health Servicea nd the AmericanC ancerS ocietyt o D.T. Denhardt,a nd from the Ulfited StatesN ationalS cienceF oundationto M. MeselsonS. .A. was a Fellow of the Damon Runyon MemoriaFl und.

PY - 1970/11/12

Y1 - 1970/11/12

N2 - 1. 1. A comparison of the actions of pancreatic deoxyribonuclease and a partially purified T4-induced endonuclease on the superhelical F{cyrillic}X174 replicative form (RF I) is presented. Pancreatic deoxyribonuclease appears to introduce single-stranded breaks into RF I without discriminating between the two strands, whereas the T4-induced endonuclease shows a pronounced preference for the "minus" strand. 2. 2. The viral or "plus" strand of RF I is normally about two and a half times as infective in spheroplasts as the complementary "minus" strand.

AB - 1. 1. A comparison of the actions of pancreatic deoxyribonuclease and a partially purified T4-induced endonuclease on the superhelical F{cyrillic}X174 replicative form (RF I) is presented. Pancreatic deoxyribonuclease appears to introduce single-stranded breaks into RF I without discriminating between the two strands, whereas the T4-induced endonuclease shows a pronounced preference for the "minus" strand. 2. 2. The viral or "plus" strand of RF I is normally about two and a half times as infective in spheroplasts as the complementary "minus" strand.

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Evidence for preferential breakage of the minus strand of ΦX174 replicative form DNA by a T4-induced endonuclease

Abstract

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