Evaluation of YadC protein delivered by live attenuated Salmonella as a vaccine against plague

Wei Sun, Joseph Olinzock, Shifeng Wang, Shilpa Sanapala, Roy Curtiss

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Yersinia pestis YadB and YadC are two new outer membrane proteins related to its pathogenicity. Here, codon-optimized yadC, yadC810 (aa 32-551), or yadBC antigen genes delivered by live attenuated Salmonella strains are evaluated in mice for induction of protective immune responses against Y. pestis CO92 through subcutaneous or intranasal challenge. Our findings indicate that mice immunized with Salmonella synthesizing YadC, YadC810, or YadBC develop significant serum IgG responses to purified recombinant YadC protein. For subcutaneous challenge (approximately 230 LD50 of Y. pestis CO92), mice immunized with Salmonella synthesizing YadC or YadC810 are afforded 50% protection, but no protection by immunization with the Salmonella strain synthesizing YadBC. None of these antigens provided protection against intranasal challenge (approximately 31 LD50 of Y. pestis CO92). In addition, subcutaneous immunization with purified YadC810 protein emulsified with alum adjuvant does not elicit a protective response against Y. pestis administered by either challenge route. Mice immunized with Salmonella vector delivering YadC were afforded partial protection against bubonic plague challenge.

Original languageEnglish (US)
Pages (from-to)119-131
Number of pages13
JournalPathogens and Disease
Volume70
Issue number2
DOIs
StatePublished - Mar 2014

Keywords

  • Bubonic plague
  • Live attenuated Salmonella vaccine
  • Pneumonic plague
  • Protective response
  • YadC
  • Yersinia pestis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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