TY - JOUR
T1 - Evaluation of toxicity and oxidative stress induced by copper oxide nanoparticles in the green alga Chlamydomonas reinhardtii
AU - Melegari, Silvia Pedroso
AU - Perreault, François
AU - Costa, Rejane Helena Ribeiro
AU - Popovic, Radovan
AU - Matias, William Gerson
N1 - Funding Information:
This work was supported by research grants awarded to W.G. Matias by the CNPq (Brazil) and to R. Popovic by the NSERC (Canada) . F. Perreault is supported by a NSERC Ph.D. fellowship . S.P. Melegari thanks the CAPES (Brazil) for a post-doctoral fellowship at UQAM. The authors thank the staff at the Faculty for Electron Microscopy Research of McGill University.
PY - 2013/10/15
Y1 - 2013/10/15
N2 - Copper oxide nanoparticles (CuO NP) are frequently employed for their antimicrobial properties in antifouling paints. Their extensive use can contaminate aquatic ecosystems. However, the toxicological effects of this NP in the environment are poorly known. In this study, we evaluated the toxicity and oxidative stress induced by CuO NP on Chlamydomonas reinhardtii using several toxicological assays. CuO NP was found to induce growth inhibition and a significant decrease in carotenoids levels. From data on cells density after 72h of CuO NP exposure in light, the EC50 value was calculated to be 150.45±1.17mgL-1 and the NOEC≤100mgL-1. Evaluation of esterase activity demonstrates a decrease in cell metabolism activity with the increase of CuO NP concentration. The CuO NP induced an increase of reactive species level (190±0.45% at 1000mgL-1 after 72h of exposition, compared to control) and lipid peroxidation of cellular membranes (73±2% at 1000mgL-1 of CuO NP in 72h of exposition, compared to control). Investigation of CuO NP uptake showed the presence of NP into C. reinhardtii cells in different sites of the cell and, biomarkers of enzymatic antioxidants showed a change of activity after CuO NP exposition. In conclusion, C. reinhardtii was shown to be sensitive to the presence of CuO NP in their environment and CuO NP treatments induced a toxic response from 0.1mgL-1 after 72h of treatment.
AB - Copper oxide nanoparticles (CuO NP) are frequently employed for their antimicrobial properties in antifouling paints. Their extensive use can contaminate aquatic ecosystems. However, the toxicological effects of this NP in the environment are poorly known. In this study, we evaluated the toxicity and oxidative stress induced by CuO NP on Chlamydomonas reinhardtii using several toxicological assays. CuO NP was found to induce growth inhibition and a significant decrease in carotenoids levels. From data on cells density after 72h of CuO NP exposure in light, the EC50 value was calculated to be 150.45±1.17mgL-1 and the NOEC≤100mgL-1. Evaluation of esterase activity demonstrates a decrease in cell metabolism activity with the increase of CuO NP concentration. The CuO NP induced an increase of reactive species level (190±0.45% at 1000mgL-1 after 72h of exposition, compared to control) and lipid peroxidation of cellular membranes (73±2% at 1000mgL-1 of CuO NP in 72h of exposition, compared to control). Investigation of CuO NP uptake showed the presence of NP into C. reinhardtii cells in different sites of the cell and, biomarkers of enzymatic antioxidants showed a change of activity after CuO NP exposition. In conclusion, C. reinhardtii was shown to be sensitive to the presence of CuO NP in their environment and CuO NP treatments induced a toxic response from 0.1mgL-1 after 72h of treatment.
KW - Antioxidants enzymes activity
KW - Chlamydomonas reinhardtii
KW - Copper oxide nanoparticles
KW - Lipid peroxidation
KW - Reactive oxygen species
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U2 - 10.1016/j.aquatox.2013.09.015
DO - 10.1016/j.aquatox.2013.09.015
M3 - Article
C2 - 24113166
AN - SCOPUS:84885213152
SN - 0166-445X
VL - 142-143
SP - 431
EP - 440
JO - Aquatic Toxicology
JF - Aquatic Toxicology
ER -