Evaluation of the influence of growth medium composition on cell elasticity

Mehdi Nikkhah, Jeannine S. Strobl, Eva M. Schmelz, Masoud Agah

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Recently, there has been an increasing interest in using the biomechanical properties of cells as biomarkers to discriminate between normal and cancerous cells. However, few investigators have considered the influence of the growth medium composition when evaluating the biomechanical properties of the normal and diseased cells. In this study, we investigated the variation in Young's modulus of non-malignant MCF10A and malignant MDA-MB-231 breast cells seeded in five different growth media under controlled experimental conditions. The average Young's modulus of MDA-MB-231 cells was significantly lower (p<0.0001) than the mean Young's modulus of MCF10A cells when compared in identical medium compositions. However, we found that growth medium composition affected the elasticity of MCF10A and MDA-MB-231 cells. The average Young's modulus of both cell lines decreased by 10-18% when the serum was reduced from 10% to 5% and upon addition of epidermal growth factor (EGF, 20. ng/ml) to the medium. Though these elasticity changes might have some biological impact, none was statistically significant. However, the elasticity of MCF10A was significantly more responsive than MDA-MB-231 cells to the medium composition supplemented with EGF, cholera toxin (CT), insulin (INS) and hydrocortisone (HC), which are recommended for routine cultivation of MCF10A cells (M5). MCF10A cells were significantly softer (p<0.002) when grown in medium M5 compared to a standard MDA-MB-231 medium (M1). The investigation of the effects of culture medium composition on the elastic properties of cells highlights the need to take these effects into consideration when interpreting elasticity measurements in cells grown in different media.

Original languageEnglish (US)
Pages (from-to)762-766
Number of pages5
JournalJournal of Biomechanics
Volume44
Issue number4
DOIs
StatePublished - Feb 24 2011
Externally publishedYes

Fingerprint

Elasticity
Elastic moduli
Growth
Chemical analysis
Elastic Modulus
Cells
Cortisol
Epidermal Growth Factor
Insulin
Biomarkers
Cholera Toxin
Culture Media
Hydrocortisone
Breast
Research Personnel

Keywords

  • AFM
  • Growth medium
  • MCF10A
  • MDA-MB-231
  • Young's modulus

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Rehabilitation
  • Biophysics
  • Biomedical Engineering

Cite this

Evaluation of the influence of growth medium composition on cell elasticity. / Nikkhah, Mehdi; Strobl, Jeannine S.; Schmelz, Eva M.; Agah, Masoud.

In: Journal of Biomechanics, Vol. 44, No. 4, 24.02.2011, p. 762-766.

Research output: Contribution to journalArticle

Nikkhah, Mehdi ; Strobl, Jeannine S. ; Schmelz, Eva M. ; Agah, Masoud. / Evaluation of the influence of growth medium composition on cell elasticity. In: Journal of Biomechanics. 2011 ; Vol. 44, No. 4. pp. 762-766.
@article{ce2af8e81eb04d42a655503135c754a5,
title = "Evaluation of the influence of growth medium composition on cell elasticity",
abstract = "Recently, there has been an increasing interest in using the biomechanical properties of cells as biomarkers to discriminate between normal and cancerous cells. However, few investigators have considered the influence of the growth medium composition when evaluating the biomechanical properties of the normal and diseased cells. In this study, we investigated the variation in Young's modulus of non-malignant MCF10A and malignant MDA-MB-231 breast cells seeded in five different growth media under controlled experimental conditions. The average Young's modulus of MDA-MB-231 cells was significantly lower (p<0.0001) than the mean Young's modulus of MCF10A cells when compared in identical medium compositions. However, we found that growth medium composition affected the elasticity of MCF10A and MDA-MB-231 cells. The average Young's modulus of both cell lines decreased by 10-18{\%} when the serum was reduced from 10{\%} to 5{\%} and upon addition of epidermal growth factor (EGF, 20. ng/ml) to the medium. Though these elasticity changes might have some biological impact, none was statistically significant. However, the elasticity of MCF10A was significantly more responsive than MDA-MB-231 cells to the medium composition supplemented with EGF, cholera toxin (CT), insulin (INS) and hydrocortisone (HC), which are recommended for routine cultivation of MCF10A cells (M5). MCF10A cells were significantly softer (p<0.002) when grown in medium M5 compared to a standard MDA-MB-231 medium (M1). The investigation of the effects of culture medium composition on the elastic properties of cells highlights the need to take these effects into consideration when interpreting elasticity measurements in cells grown in different media.",
keywords = "AFM, Growth medium, MCF10A, MDA-MB-231, Young's modulus",
author = "Mehdi Nikkhah and Strobl, {Jeannine S.} and Schmelz, {Eva M.} and Masoud Agah",
year = "2011",
month = "2",
day = "24",
doi = "10.1016/j.jbiomech.2010.11.002",
language = "English (US)",
volume = "44",
pages = "762--766",
journal = "Journal of Biomechanics",
issn = "0021-9290",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Evaluation of the influence of growth medium composition on cell elasticity

AU - Nikkhah, Mehdi

AU - Strobl, Jeannine S.

AU - Schmelz, Eva M.

AU - Agah, Masoud

PY - 2011/2/24

Y1 - 2011/2/24

N2 - Recently, there has been an increasing interest in using the biomechanical properties of cells as biomarkers to discriminate between normal and cancerous cells. However, few investigators have considered the influence of the growth medium composition when evaluating the biomechanical properties of the normal and diseased cells. In this study, we investigated the variation in Young's modulus of non-malignant MCF10A and malignant MDA-MB-231 breast cells seeded in five different growth media under controlled experimental conditions. The average Young's modulus of MDA-MB-231 cells was significantly lower (p<0.0001) than the mean Young's modulus of MCF10A cells when compared in identical medium compositions. However, we found that growth medium composition affected the elasticity of MCF10A and MDA-MB-231 cells. The average Young's modulus of both cell lines decreased by 10-18% when the serum was reduced from 10% to 5% and upon addition of epidermal growth factor (EGF, 20. ng/ml) to the medium. Though these elasticity changes might have some biological impact, none was statistically significant. However, the elasticity of MCF10A was significantly more responsive than MDA-MB-231 cells to the medium composition supplemented with EGF, cholera toxin (CT), insulin (INS) and hydrocortisone (HC), which are recommended for routine cultivation of MCF10A cells (M5). MCF10A cells were significantly softer (p<0.002) when grown in medium M5 compared to a standard MDA-MB-231 medium (M1). The investigation of the effects of culture medium composition on the elastic properties of cells highlights the need to take these effects into consideration when interpreting elasticity measurements in cells grown in different media.

AB - Recently, there has been an increasing interest in using the biomechanical properties of cells as biomarkers to discriminate between normal and cancerous cells. However, few investigators have considered the influence of the growth medium composition when evaluating the biomechanical properties of the normal and diseased cells. In this study, we investigated the variation in Young's modulus of non-malignant MCF10A and malignant MDA-MB-231 breast cells seeded in five different growth media under controlled experimental conditions. The average Young's modulus of MDA-MB-231 cells was significantly lower (p<0.0001) than the mean Young's modulus of MCF10A cells when compared in identical medium compositions. However, we found that growth medium composition affected the elasticity of MCF10A and MDA-MB-231 cells. The average Young's modulus of both cell lines decreased by 10-18% when the serum was reduced from 10% to 5% and upon addition of epidermal growth factor (EGF, 20. ng/ml) to the medium. Though these elasticity changes might have some biological impact, none was statistically significant. However, the elasticity of MCF10A was significantly more responsive than MDA-MB-231 cells to the medium composition supplemented with EGF, cholera toxin (CT), insulin (INS) and hydrocortisone (HC), which are recommended for routine cultivation of MCF10A cells (M5). MCF10A cells were significantly softer (p<0.002) when grown in medium M5 compared to a standard MDA-MB-231 medium (M1). The investigation of the effects of culture medium composition on the elastic properties of cells highlights the need to take these effects into consideration when interpreting elasticity measurements in cells grown in different media.

KW - AFM

KW - Growth medium

KW - MCF10A

KW - MDA-MB-231

KW - Young's modulus

UR - http://www.scopus.com/inward/record.url?scp=79951721440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951721440&partnerID=8YFLogxK

U2 - 10.1016/j.jbiomech.2010.11.002

DO - 10.1016/j.jbiomech.2010.11.002

M3 - Article

VL - 44

SP - 762

EP - 766

JO - Journal of Biomechanics

JF - Journal of Biomechanics

SN - 0021-9290

IS - 4

ER -