Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA

Ascención Torres-Escobar; María Dolores Juárez-Rodríguez; Christine G. Branger; Roy Curtiss

doi:10.1016/j.vaccine.2010.06.070

Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA

Ascención Torres-Escobar, María Dolores Juárez-Rodríguez, Christine G. Branger, Roy Curtiss

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.

Original language	English (US)
Pages (from-to)	5810-5816
Number of pages	7
Journal	Vaccine
Volume	28
Issue number	36
DOIs	https://doi.org/10.1016/j.vaccine.2010.06.070
State	Published - Aug 2010

Keywords

Asd
Codon-optimized
PsaA antigen
PsaB chaperone protein
PsaC usher protein
Vaccine plasmid

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2010.06.070

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Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA. / Torres-Escobar, Ascención; Juárez-Rodríguez, María Dolores; Branger, Christine G. et al.
In: Vaccine, Vol. 28, No. 36, 08.2010, p. 5810-5816.

Research output: Contribution to journal › Article › peer-review

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abstract = "Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.",

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AU - Branger, Christine G.

AU - Curtiss, Roy

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Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA

Abstract

Keywords

ASJC Scopus subject areas

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