Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA

Ascención Torres-Escobar, María Dolores Juárez-Rodríguez, Christine G. Branger, Roy Curtiss

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.

Original languageEnglish (US)
Pages (from-to)5810-5816
Number of pages7
JournalVaccine
Volume28
Issue number36
DOIs
StatePublished - Aug 2010

Fingerprint

Yersinia pestis
Salmonella enterica
Humoral Immunity
humoral immunity
Salmonella Vaccines
Attenuated Vaccines
Synthetic Vaccines
mice
Salmonella
vaccines
oral vaccination
adhesins
Plague
plague
immunoglobulin G
lethal genes
codons
Codon
Immunization
macrophages

Keywords

  • Asd
  • Codon-optimized
  • PsaA antigen
  • PsaB chaperone protein
  • PsaC usher protein
  • Vaccine plasmid

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA. / Torres-Escobar, Ascención; Juárez-Rodríguez, María Dolores; Branger, Christine G.; Curtiss, Roy.

In: Vaccine, Vol. 28, No. 36, 08.2010, p. 5810-5816.

Research output: Contribution to journalArticle

Torres-Escobar, Ascención ; Juárez-Rodríguez, María Dolores ; Branger, Christine G. ; Curtiss, Roy. / Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA. In: Vaccine. 2010 ; Vol. 28, No. 36. pp. 5810-5816.
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