Evaluation of the humoral immune response in mice orally vaccinated with live recombinant attenuated Salmonella enterica delivering a secreted form of Yersinia pestis PsaA

Ascención Torres-Escobar, María Dolores Juárez-Rodríguez, Christine G. Branger, Roy Curtiss

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.

Original languageEnglish (US)
Pages (from-to)5810-5816
Number of pages7
JournalVaccine
Volume28
Issue number36
DOIs
StatePublished - Aug 2010

Keywords

  • Asd
  • Codon-optimized
  • PsaA antigen
  • PsaB chaperone protein
  • PsaC usher protein
  • Vaccine plasmid

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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