Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0

Po-Lin Chiu, Xueming Li, Zongli Li, Brian Beckett, Axel F. Brilot, Nikolaus Grigorieff, David A. Agard, Yifan Cheng, Thomas Walz

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.

Original languageEnglish (US)
Pages (from-to)163-173
Number of pages11
JournalJournal of Structural Biology
Volume192
Issue number2
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

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Keywords

  • Direct electron detection device camera
  • Electron counting
  • Super-resolution
  • Two-dimensional crystal

ASJC Scopus subject areas

  • Structural Biology

Cite this

Chiu, P-L., Li, X., Li, Z., Beckett, B., Brilot, A. F., Grigorieff, N., Agard, D. A., Cheng, Y., & Walz, T. (2015). Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0. Journal of Structural Biology, 192(2), 163-173. https://doi.org/10.1016/j.jsb.2015.08.015