Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0

Po Lin Chiu; Xueming Li; Zongli Li; Brian Beckett; Axel F. Brilot; Nikolaus Grigorieff; David A. Agard; Yifan Cheng; Thomas Walz

doi:10.1016/j.jsb.2015.08.015

Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0

Po Lin Chiu, Xueming Li, Zongli Li, Brian Beckett, Axel F. Brilot, Nikolaus Grigorieff, David A. Agard, Yifan Cheng, Thomas Walz

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.

Original language	English (US)
Pages (from-to)	163-173
Number of pages	11
Journal	Journal of Structural Biology
Volume	192
Issue number	2
DOIs	https://doi.org/10.1016/j.jsb.2015.08.015
State	Published - Nov 2015
Externally published	Yes

Keywords

Direct electron detection device camera
Electron counting
Super-resolution
Two-dimensional crystal

ASJC Scopus subject areas

Structural Biology

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10.1016/j.jsb.2015.08.015

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abstract = "The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.",

keywords = "Direct electron detection device camera, Electron counting, Super-resolution, Two-dimensional crystal",

author = "Chiu, {Po Lin} and Xueming Li and Zongli Li and Brian Beckett and Brilot, {Axel F.} and Nikolaus Grigorieff and Agard, {David A.} and Yifan Cheng and Thomas Walz",

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year = "2015",

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doi = "10.1016/j.jsb.2015.08.015",

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AU - Chiu, Po Lin

AU - Li, Xueming

AU - Li, Zongli

AU - Beckett, Brian

AU - Brilot, Axel F.

AU - Grigorieff, Nikolaus

AU - Agard, David A.

AU - Cheng, Yifan

AU - Walz, Thomas

PY - 2015/11

Y1 - 2015/11

N2 - The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.

AB - The K2 Summit camera was initially the only commercially available direct electron detection camera that was optimized for high-speed counting of primary electrons and was also the only one that implemented centroiding so that the resolution of the camera can be extended beyond the Nyquist limit set by the physical pixel size. In this study, we used well-characterized two-dimensional crystals of the membrane protein aquaporin-0 to characterize the performance of the camera below and beyond the physical Nyquist limit and to measure the influence of electron dose rate on image amplitudes and phases.

KW - Direct electron detection device camera

KW - Electron counting

KW - Super-resolution

KW - Two-dimensional crystal

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Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0

Abstract

Keywords

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