Evaluation of SIV library vaccines with genetic cytokines in a macaque challenge

Kathryn F. Sykes, Mark G. Lewis, Burke Squires, Stephen Johnston

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Gene and expression library immunization make it possible to functionally test all the gene-encoded antigens of a pathogen in a host challenge system. This comprehensive method could generate new and better vaccine candidates. We constructed expression libraries from simian immunodeficiency virus (SIV) cDNA and genetically immunize monkeys with the libraries alone or with a low dose of plasmids encoding human IL-12 and GMCSF. Eight of twelve animals in the three test groups showed some anti-SIV immune response, whereas the controls did not. Six months after priming, monkeys were intravenously challenged with virulent SIVmac251. All were infected but animals in two groups vaccinated with SIV libraries showed a trend toward lower viral-loads, mitigated clinical disease, and higher survival rates than controls. Significantly, co-administering the GMCSF and IL-12-encoding plasmids worsened these measures of protection. This preliminary study should encourage further development of library-vaccine strategies and caution the use of cytokines as adjuvants.

Original languageEnglish (US)
Pages (from-to)2382-2395
Number of pages14
JournalVaccine
Volume20
Issue number17-18
DOIs
StatePublished - May 22 2002
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca
cytokines
Vaccines
Interleukin-12
Cytokines
vaccines
Libraries
Haplorhini
Plasmids
interleukin-12
monkeys
Viral Load
plasmids
Gene Library
Immunization
Complementary DNA
Gene Expression
Antigens

Keywords

  • ELI
  • Genetic adjuvants
  • SIV trial

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Evaluation of SIV library vaccines with genetic cytokines in a macaque challenge. / Sykes, Kathryn F.; Lewis, Mark G.; Squires, Burke; Johnston, Stephen.

In: Vaccine, Vol. 20, No. 17-18, 22.05.2002, p. 2382-2395.

Research output: Contribution to journalArticle

Sykes, Kathryn F. ; Lewis, Mark G. ; Squires, Burke ; Johnston, Stephen. / Evaluation of SIV library vaccines with genetic cytokines in a macaque challenge. In: Vaccine. 2002 ; Vol. 20, No. 17-18. pp. 2382-2395.
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