Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol

M. J. Huber-smith; R. Nesse; M. Mazhar; D. S. McCann

doi:10.1016/S0378-4347(00)80764-1

Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol

M. J. Huber-smith, R. Nesse, M. Mazhar, D. S. McCann

CLAS-NS: Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [³H]MHPG. The HPLC procedure utilizes a C₁₈ column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).

Original language	English (US)
Pages (from-to)	91-99
Number of pages	9
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	377
Issue number	C
DOIs	https://doi.org/10.1016/S0378-4347(00)80764-1
State	Published - 1986

ASJC Scopus subject areas

Chemistry(all)

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@article{ccebe36ffed0428dae3b71ee85eb1ae8,

title = "Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol",

abstract = "A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).",

author = "Huber-smith, {M. J.} and R. Nesse and M. Mazhar and McCann, {D. S.}",

year = "1986",

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T1 - Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol

AU - Huber-smith, M. J.

AU - Nesse, R.

AU - Mazhar, M.

AU - McCann, D. S.

PY - 1986

Y1 - 1986

N2 - A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).

AB - A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).

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