Abstract
A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 91-99 |
| Number of pages | 9 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 377 |
| Issue number | C |
| DOIs | |
| State | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Clinical Biochemistry
- Molecular Medicine
Cite this
Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol. / Huber-smith, M. J.; Nesse, Randolph; Mazhar, M.; McCann, D. S.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 377, No. C, 1986, p. 91-99.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol
AU - Huber-smith, M. J.
AU - Nesse, Randolph
AU - Mazhar, M.
AU - McCann, D. S.
PY - 1986
Y1 - 1986
N2 - A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).
AB - A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).
UR - http://www.scopus.com/inward/record.url?scp=0023056677&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023056677&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)80764-1
DO - 10.1016/S0378-4347(00)80764-1
M3 - Article
C2 - 3711248
AN - SCOPUS:0023056677
VL - 377
SP - 91
EP - 99
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
SN - 0378-4347
IS - C
ER -