Evaluation of plasma 3-methoxy-4-hydroxyphenylglycol

M. J. Huber-smith, R. Nesse, M. Mazhar, D. S. McCann

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Abstract

A high-performance liquid chromatographic (HPLC) assay suitable for the evaluation of 3-methoxy-4-hydroxyphenylglycol (MHPG) in 1-ml aliquots of plasma is presented. Preparatory steps include extraction into ethyl acetate and minicolumn chromatography. Recoveries are monitored with [3H]MHPG. The HPLC procedure utilizes a C18 column, isocratic elution and amperometric detection. The assay was checked against a gas chromatographic-mass spectrometric procedure; the two procedures correlated well with a correlation coefficient of 0.99. Intra-assay reproducibility was 5.4%, inter-assay reproducibility 10.7%. Immediate changes in the orthostatic position did not affect the plasma MHPG concentration. Based on 22 normal controls the daytime plasma MHPG level was 2.98 ± 0.66 ng/ml (mean ± S.D.).

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume377
Issue numberC
DOIs
StatePublished - 1986
Externally publishedYes

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ASJC Scopus subject areas

  • Chemistry(all)

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