Evaluation of drug carrier hepatotoxicity using primary cell culture models

Güneş Kibar, Subhadeep Dutta, Kaushal Rege, O. Berk Usta

Research output: Contribution to journalArticlepeer-review


This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations <50 μg/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC50 value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physicochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.

Original languageEnglish (US)
Article number102651
JournalNanomedicine: Nanotechnology, Biology, and Medicine
StatePublished - Feb 2023


  • Hepatotoxicity
  • In vitro culture
  • Lipopolymer nanoparticle (LPN)
  • Nanotoxicity
  • Primary rat hepatocyte

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science


Dive into the research topics of 'Evaluation of drug carrier hepatotoxicity using primary cell culture models'. Together they form a unique fingerprint.

Cite this