TY - JOUR
T1 - Evaluation of cell-disruption effects of pulsed-electric-field treatment of Synechocystis PCC 6803
AU - Sheng, Jie
AU - Vannela, Raveender
AU - Rittmann, Bruce
PY - 2011/4/15
Y1 - 2011/4/15
N2 - In order to use Synechocystis PCC 6803 as feedstock of nonpetroleum-based diesel fuel, pulsed electric field (PEF) technology was used for cell disruption prior to extraction of intracellular lipids. Severe cell disruption was evident after PEF treatment, especially with treatment intensity (TI) > 35 kWh/m3. Temperature increase during the treatment brought about most of the destruction of autofluorescence compounds, as well as a fraction of inactivation and the destruction of the plasma and thylakoid membranes. However, the forces associated with the pulsing electric field caused significant damage to the plasma membrane, cell wall, and thylakoid membrane, and it even led to complete disruption of some cells into fragments, which resulted in biomass loss. Treatment by PEF enhanced the potential for the low-toxicity solvent isopropanol to access lipid molecules during subsequent solvent extraction, leading to lower usage of isopropanol for the same extraction efficiency. Thus, PEF shows promise for lowering the costs and environmental effects of the lipid-extraction step.
AB - In order to use Synechocystis PCC 6803 as feedstock of nonpetroleum-based diesel fuel, pulsed electric field (PEF) technology was used for cell disruption prior to extraction of intracellular lipids. Severe cell disruption was evident after PEF treatment, especially with treatment intensity (TI) > 35 kWh/m3. Temperature increase during the treatment brought about most of the destruction of autofluorescence compounds, as well as a fraction of inactivation and the destruction of the plasma and thylakoid membranes. However, the forces associated with the pulsing electric field caused significant damage to the plasma membrane, cell wall, and thylakoid membrane, and it even led to complete disruption of some cells into fragments, which resulted in biomass loss. Treatment by PEF enhanced the potential for the low-toxicity solvent isopropanol to access lipid molecules during subsequent solvent extraction, leading to lower usage of isopropanol for the same extraction efficiency. Thus, PEF shows promise for lowering the costs and environmental effects of the lipid-extraction step.
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U2 - 10.1021/es103339x
DO - 10.1021/es103339x
M3 - Article
C2 - 21428280
AN - SCOPUS:79954536976
SN - 0013-936X
VL - 45
SP - 3795
EP - 3802
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 8
ER -