Evaluation of biological sample preparation for immunosignature-based diagnostics

Brian Andrew Chase, Stephen Johnston, Joseph Barten Legutki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To address the need for a universal system to assess health status, we previously described a method termed "immunosignaturing"which splays the entire humoral antibody repertoire across a peptide microarray. Two important issues relative to the potential broad use of immunosignatures are sample preparation and stability. In the present study, we compared the immunosignatures developed from serum, plasma, saliva, and antibodies eluted from blood dried onto filter paper. We found that serum and plasma provide identical immunosignatures. Immunosignatures derived from dried blood also correlated well with those from nondried serum from the same individual. Immunosignatures derived from dried blood were capable of distinguishing naïve mice from those infected with influenza virus. Saliva was applied to the arrays, and the IgA immunosignature correlated strongly with that from dried blood. Finally, we demonstrate that dried blood retains immunosignature information even when exposed to high temperature. This work expands the potential diagnostic uses for immunosignatures. These features suggest that different forms of archival samples can be used for diagnosis development and that in prospective studies samples can be easily procured.

Original languageEnglish (US)
Pages (from-to)352-358
Number of pages7
JournalClinical and Vaccine Immunology
Volume19
Issue number3
DOIs
StatePublished - Mar 2012

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Blood
Saliva
Serum
Plasmas
Antibodies
Microarrays
Orthomyxoviridae
Viruses
Immunoglobulin A
Health Status
Health
Prospective Studies
Peptides
Temperature

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Evaluation of biological sample preparation for immunosignature-based diagnostics. / Chase, Brian Andrew; Johnston, Stephen; Legutki, Joseph Barten.

In: Clinical and Vaccine Immunology, Vol. 19, No. 3, 03.2012, p. 352-358.

Research output: Contribution to journalArticle

Chase, Brian Andrew ; Johnston, Stephen ; Legutki, Joseph Barten. / Evaluation of biological sample preparation for immunosignature-based diagnostics. In: Clinical and Vaccine Immunology. 2012 ; Vol. 19, No. 3. pp. 352-358.
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