Evaluating the spatial and temporal protein production in neural tissue engineering constructs in vitro

Caroline P. Addington, Christine Pauken, Sarah Stabenfeldt

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tissue engineering constructs are uniquely positioned not only for in vivo regenerative therapeutic applications but also to evaluate cellular responses to modifications in finely tuned three-dimensional (3-D) microenvironments as opposed to traditional two-dimensional (2-D) culture systems. However, moving into a 3-D system presents complications of assessing spatial and temporal alterations in cellular behavior (e.g., protein synthesis, motility, etc.). Here, we describe methods to evaluate spatial and temporal protein production in neural tissue engineering construct.

Original languageEnglish (US)
Title of host publicationExtracellular Matrix
PublisherSpringer New York
Pages163-179
Number of pages17
ISBN (Print)9781493920839, 9781493920822
DOIs
StatePublished - Nov 3 2014

Keywords

  • Cryosectioning
  • Immunocytochemistry
  • Neural tissue engineering
  • Western blot

ASJC Scopus subject areas

  • Medicine(all)
  • Neuroscience(all)
  • Agricultural and Biological Sciences(all)

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