Evaluating the spatial and temporal protein production in neural tissue engineering constructs in vitro

Caroline P. Addington; Christine Pauken; Sarah Stabenfeldt

doi:10.1007/978-1-4939-2083-9_14

Evaluating the spatial and temporal protein production in neural tissue engineering constructs in vitro

Caroline P. Addington, Christine Pauken, Sarah Stabenfeldt

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Tissue engineering constructs are uniquely positioned not only for in vivo regenerative therapeutic applications but also to evaluate cellular responses to modifications in finely tuned three-dimensional (3-D) microenvironments as opposed to traditional two-dimensional (2-D) culture systems. However, moving into a 3-D system presents complications of assessing spatial and temporal alterations in cellular behavior (e.g., protein synthesis, motility, etc.). Here, we describe methods to evaluate spatial and temporal protein production in neural tissue engineering construct.

Original language	English (US)
Title of host publication	Extracellular Matrix
Publisher	Springer New York
Pages	163-179
Number of pages	17
ISBN (Electronic)	9781493920839
ISBN (Print)	9781493920822
DOIs	https://doi.org/10.1007/978-1-4939-2083-9_14
State	Published - Nov 3 2014

Keywords

Cryosectioning
Immunocytochemistry
Neural tissue engineering
Western blot

ASJC Scopus subject areas

General Medicine
General Neuroscience
General Agricultural and Biological Sciences

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PY - 2014/11/3

Y1 - 2014/11/3

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KW - Western blot

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Evaluating the spatial and temporal protein production in neural tissue engineering constructs in vitro

Abstract

Keywords

ASJC Scopus subject areas

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