Estrogen receptor alpha containing neurons in the monkey forebrain: Lack of association with calcium binding proteins and choline acetyltransferase

Sylvia Perez, T. J. Sendera, J. H. Kordower, E. J. Mufson

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The present study used single and dual immunohistochemistry to determine the topography and chemical phenotype of ERα containing neurons within the monkey forebrain utilizing antibodies directed against the full-length human ERα (NCL-ER-6F11), calcium-binding proteins calbindin-D28k, and parvalbumin as well as choline acetyltransferase (ChAT). Our findings demonstrate for the first time ERα immunoreactive (-ir) cells in the monkey cerebral cortex (layers I-II) and in the claustrum. In addition, ERα-ir cells were seen in the septum, basal forebrain, amygdala and hypothalamus. Double-labeled cells for ERα and calbindin-D28k were seen only in the ventrolateral part of the ventromedial hypothalamic nucleus. In contrast, the co-localization of ERα and parvalbumin or ChAT was not seen in any of the areas of the monkey forebrain examined. These observations suggest that estrogens, at least in part, via ERα regulate calbindin-D28k hypothalamic but not parvalbumin or ChAT containing neurons in select monkey forebrain regions.

Original languageEnglish (US)
Pages (from-to)55-63
Number of pages9
JournalBrain Research
Volume1019
Issue number1-2
DOIs
StatePublished - Sep 3 2004
Externally publishedYes

Keywords

  • Brain
  • Calbindin
  • Cholinergic
  • Estrogen
  • Immunohistochemistry
  • Neurotransmitters, modulators, transporters, receptors
  • Parvalbumin
  • Regional localization of receptors and transmitters

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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