Estrogen mimics induce genes encoding chemical efflux proteins in gram-negative bacteria

Xinhua Li, Sondra Teske, Otakuye Conroy-Ben

Research output: Contribution to journalArticle

Abstract

Escherichia coli and Pseudomonas aeruginosa are gram-negative bacteria found in wastewater and biosolids. Spanning the inner and outer membrane are resistance-nodulation-cell division superfamily (RND) efflux pumps responsible for detoxification of the cell, typically in response to antibiotics and other toxicity inducing substrates. Here, we show that estrogenic endocrine disruptors, common wastewater pollutants, induce genes encoding chemical efflux proteins. Bacteria were exposed to environmental concentrations of the synthetic estrogen 17α-ethynylestradiol, the surfactant nonylphenol, and the plasticizer bisphenol-A, and analyzed for RND gene expression via q-PCR. Results showed that the genes acrB and yhiV were over-expressed in response to the three chemicals in E. coli, and support previous findings that these two transporters export hormones. P. aeruginosa contains 12 RND efflux pumps, which were differentially expressed in response to the three chemicals: 17α-ethynylestradiol, bisphenol-A, and nonylphenol up-regulated mexD and mexF, while nonylphenol and bisphenol-A positively affected transcription of mexK, mexW, and triC. Gene expression via q-PCR of RND genes may be used to predict the interaction of estrogen mimics with RND genes. One bacterial response to estrogen mimic exposure is to induce gene expression of chemical efflux proteins, which leads to the expulsion of the contaminant from the cell.

Original languageEnglish (US)
Pages (from-to)327-331
Number of pages5
JournalChemosphere
Volume128
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

Fingerprint

Gene encoding
nodulation
Gram-Negative Bacteria
Cell Division
Bacteria
Estrogens
Gene expression
Proteins
bacterium
protein
gene
Genes
gene expression
Escherichia coli
Waste Water
Wastewater
Gene Expression
Pseudomonas aeruginosa
Pumps
Estradiol Congeners

Keywords

  • Efflux proteins
  • Estrogenic endocrine disruptors
  • Gram-negative bacteria
  • Q-PCR
  • RND

ASJC Scopus subject areas

  • Environmental Chemistry
  • Chemistry(all)

Cite this

Estrogen mimics induce genes encoding chemical efflux proteins in gram-negative bacteria. / Li, Xinhua; Teske, Sondra; Conroy-Ben, Otakuye.

In: Chemosphere, Vol. 128, 01.06.2015, p. 327-331.

Research output: Contribution to journalArticle

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