Estimating the cellular maintenance coefficient and its use in the design of two-phase partitioning bioscrubbers

Sung Ho Yeom, Andrew J. Daugulis, David Nielsen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

One of the key roles of an organic solvent has emerged to be the enhancement of oxygen transfer in two-phase partitioning bioscrubbers (TPPBs). In order to determine an optimum organic fraction for a given VOCs loading, the oxygen demand of the total cell mass must be estimated, which depends upon the magnitude of the cellular maintenance coefficient. We have estimated the dynamics of the maintenance coefficient for benzene degradation by Achromobacter xylosoxidans Y234 in a TPPB and found that the maintenance coefficient generally decreased as cells accumulated in the TPPB but converged to a specific value of 1.750 × 10-2 h-1 at biological steady state. Due to its important influence on all of the essential design parameters of the TPPB system, including optimum organic fraction, aeration rate and agitation speed, the maintenance coefficient should be considered as a key biological determinant for microorganism selection, as well as in overall TPPB design.

Original languageEnglish (US)
Pages (from-to)731-739
Number of pages9
JournalBioprocess and biosystems engineering
Volume33
Issue number6
DOIs
StatePublished - Aug 1 2010

Keywords

  • Benzene
  • Cell mass
  • Loading rate
  • Maintenance coefficient
  • Oxygen requirement
  • Two-phase partitioning bioscrubber

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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