Establishment of lymphoblastoid cell lines from Marek's disease primary tumors.

X. Liu; L. F. Lee; J. M. Sharma; K. Nazerian

doi:10.3382/ps.0621902

Establishment of lymphoblastoid cell lines from Marek's disease primary tumors.

X. Liu, L. F. Lee, J. M. Sharma, K. Nazerian

Immunotherapy, Vaccines and Virotherapy, Center for (CIVV)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Seven lymphoblastoid cell lines were developed from 15 Marek's disease primary lymphomas treated to remove adherent cells (46.7% success rate) and 1 cell line was developed from 15 untreated lymphomas (6.7% success rate). These cell lines are designated as MDCC-RP22 through MDCC-RP29. Tumor materials were from 151(5) x 7(1) chickens infected with either JM-111S clone or the GA-22 clone of Marek's disease virus. Factors contributing to the establishment of cell lines include, in addition to the removal of adherent cells at the initial stage of culture, the periodic adjustment and the concentration of viable cells, including the removal of dead cells. The cell lines bear T-cell surface markers and Marek's disease tumor associated surface antigen (MATSA). MDCC-RP22 and MDCC-RP23 are high virus producers.

Original language	English (US)
Pages (from-to)	1902-1905
Number of pages	4
Journal	Poultry science
Volume	62
Issue number	9
DOIs	https://doi.org/10.3382/ps.0621902
State	Published - Sep 1983

ASJC Scopus subject areas

Animal Science and Zoology

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10.3382/ps.0621902

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title = "Establishment of lymphoblastoid cell lines from Marek's disease primary tumors.",

abstract = "Seven lymphoblastoid cell lines were developed from 15 Marek's disease primary lymphomas treated to remove adherent cells (46.7% success rate) and 1 cell line was developed from 15 untreated lymphomas (6.7% success rate). These cell lines are designated as MDCC-RP22 through MDCC-RP29. Tumor materials were from 151(5) x 7(1) chickens infected with either JM-111S clone or the GA-22 clone of Marek's disease virus. Factors contributing to the establishment of cell lines include, in addition to the removal of adherent cells at the initial stage of culture, the periodic adjustment and the concentration of viable cells, including the removal of dead cells. The cell lines bear T-cell surface markers and Marek's disease tumor associated surface antigen (MATSA). MDCC-RP22 and MDCC-RP23 are high virus producers.",

author = "X. Liu and Lee, {L. F.} and Sharma, {J. M.} and K. Nazerian",

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T1 - Establishment of lymphoblastoid cell lines from Marek's disease primary tumors.

AU - Liu, X.

AU - Lee, L. F.

AU - Sharma, J. M.

AU - Nazerian, K.

N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine

PY - 1983/9

Y1 - 1983/9

N2 - Seven lymphoblastoid cell lines were developed from 15 Marek's disease primary lymphomas treated to remove adherent cells (46.7% success rate) and 1 cell line was developed from 15 untreated lymphomas (6.7% success rate). These cell lines are designated as MDCC-RP22 through MDCC-RP29. Tumor materials were from 151(5) x 7(1) chickens infected with either JM-111S clone or the GA-22 clone of Marek's disease virus. Factors contributing to the establishment of cell lines include, in addition to the removal of adherent cells at the initial stage of culture, the periodic adjustment and the concentration of viable cells, including the removal of dead cells. The cell lines bear T-cell surface markers and Marek's disease tumor associated surface antigen (MATSA). MDCC-RP22 and MDCC-RP23 are high virus producers.

AB - Seven lymphoblastoid cell lines were developed from 15 Marek's disease primary lymphomas treated to remove adherent cells (46.7% success rate) and 1 cell line was developed from 15 untreated lymphomas (6.7% success rate). These cell lines are designated as MDCC-RP22 through MDCC-RP29. Tumor materials were from 151(5) x 7(1) chickens infected with either JM-111S clone or the GA-22 clone of Marek's disease virus. Factors contributing to the establishment of cell lines include, in addition to the removal of adherent cells at the initial stage of culture, the periodic adjustment and the concentration of viable cells, including the removal of dead cells. The cell lines bear T-cell surface markers and Marek's disease tumor associated surface antigen (MATSA). MDCC-RP22 and MDCC-RP23 are high virus producers.

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Establishment of lymphoblastoid cell lines from Marek's disease primary tumors.

Abstract

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