Erratum: A transient reporter for editing enrichment (TREE) in human cells (Nucleic Acids Research(2019)47: 19(E120)Doi: 10.1093/nar/gkz713)

Kylie Standage-Beier, Stefan J. Tekel, Nicholas Brookhouser, Grace Schwarz, Toan Nguyen, Xiao Wang, David A. Brafman

Research output: Contribution to journalComment/debatepeer-review

Abstract

The reference to a recent article has been added to the Introduction: . . . Some progress has been made to help enrich for edited cells, such as co-transfecting plasmids with a fluorescent reporter and using flow cytometry to isolate reporter-positive cells. Similarly, base editors fused to fluorescent proteins have been used to enrich for edited cell populations (16,58). . . 58. Coelho M.A., Li S., Pane L.S., Firth M., Ciotta G., Wrigley J.D., Cuomo M.E., Maresca M., Taylor B.J.M. (2018) BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B. BMC Biol. 16:150. This addition does not affect the results and conclusion of the study. May 2020: an additional correction was made to remove the sentence "However, these techniques are only reporters of transfection (RoT) and do not report on base editing activity within a cell population."from the introduction. This change has been made online but is not reflected in the print version of either the original article (https://doi.org/10.1093/nar/gkz713) or the corrigendum (https://doi.org/10.1093/nar/gkaa027).

Original languageEnglish (US)
Pages (from-to)1602
Number of pages1
JournalNucleic acids research
Volume48
Issue number3
DOIs
StatePublished - 2021
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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