EPR measurements on the effects of bicarbonate and triazine resistance on the acceptor side of Photosystem II

Wim F.J. Vermaas, A. William Rutherford

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

CO2 depletion leads to an approximately 10-fold increase in the light-induced EPR signal at g = 1.82, attributed to the QA- · Fe2+ complex, in Photosystem II-enriched thylakoid membrane fragments. Upon reconstitution with HCO3-the signal decreases to the size in control samples. The split pheophytin- signal is broader in control or reconstituted than in CO2-depleted samples. It is concluded that HCO2- strongly influences the localization and conformation of the QA- · Fe+ complex. The QA- · Fe2+ and split pheophytirr- EPR signals from triazine-resistant Brassica napus were virtually identical to those from triazine-susceptible samples, indicating that the change in the 32-kDa azidoatrazine-binding protein does not lead to a confonnational change of the Qa- · Fe2+ complex.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalFEBS Letters
Volume175
Issue number2
DOIs
StatePublished - Oct 1 1984
Externally publishedYes

Keywords

  • Bicarbonate effect
  • Herbicide
  • Photosynthesis
  • Photosystem II
  • Semiquinone-iron complex
  • Triazine resistance

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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