EPR measurements on the effects of bicarbonate and triazine resistance on the acceptor side of Photosystem II

Willem Vermaas, A. William Rutherford

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

CO2 depletion leads to an approximately 10-fold increase in the light-induced EPR signal at g = 1.82, attributed to the QA - · Fe2+ complex, in Photosystem II-enriched thylakoid membrane fragments. Upon reconstitution with HCO3 -the signal decreases to the size in control samples. The split pheophytin- signal is broader in control or reconstituted than in CO2-depleted samples. It is concluded that HCO2 - strongly influences the localization and conformation of the QA - · Fe+ complex. The QA - · Fe2+ and split pheophytirr- EPR signals from triazine-resistant Brassica napus were virtually identical to those from triazine-susceptible samples, indicating that the change in the 32-kDa azidoatrazine-binding protein does not lead to a confonnational change of the Qa - · Fe2+ complex.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalFEBS Letters
Volume175
Issue number2
DOIs
StatePublished - Oct 1 1984
Externally publishedYes

Fingerprint

Triazines
Photosystem II Protein Complex
Bicarbonates
Paramagnetic resonance
Pheophytins
Brassica napus
Thylakoids
Conformations
Carrier Proteins
Membranes
Light

Keywords

  • Bicarbonate effect
  • Herbicide
  • Photosynthesis
  • Photosystem II
  • Semiquinone-iron complex
  • Triazine resistance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

EPR measurements on the effects of bicarbonate and triazine resistance on the acceptor side of Photosystem II. / Vermaas, Willem; Rutherford, A. William.

In: FEBS Letters, Vol. 175, No. 2, 01.10.1984, p. 243-248.

Research output: Contribution to journalArticle

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AU - Rutherford, A. William

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N2 - CO2 depletion leads to an approximately 10-fold increase in the light-induced EPR signal at g = 1.82, attributed to the QA - · Fe2+ complex, in Photosystem II-enriched thylakoid membrane fragments. Upon reconstitution with HCO3 -the signal decreases to the size in control samples. The split pheophytin- signal is broader in control or reconstituted than in CO2-depleted samples. It is concluded that HCO2 - strongly influences the localization and conformation of the QA - · Fe+ complex. The QA - · Fe2+ and split pheophytirr- EPR signals from triazine-resistant Brassica napus were virtually identical to those from triazine-susceptible samples, indicating that the change in the 32-kDa azidoatrazine-binding protein does not lead to a confonnational change of the Qa - · Fe2+ complex.

AB - CO2 depletion leads to an approximately 10-fold increase in the light-induced EPR signal at g = 1.82, attributed to the QA - · Fe2+ complex, in Photosystem II-enriched thylakoid membrane fragments. Upon reconstitution with HCO3 -the signal decreases to the size in control samples. The split pheophytin- signal is broader in control or reconstituted than in CO2-depleted samples. It is concluded that HCO2 - strongly influences the localization and conformation of the QA - · Fe+ complex. The QA - · Fe2+ and split pheophytirr- EPR signals from triazine-resistant Brassica napus were virtually identical to those from triazine-susceptible samples, indicating that the change in the 32-kDa azidoatrazine-binding protein does not lead to a confonnational change of the Qa - · Fe2+ complex.

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