Epigenetic (re)programming of caste-specific behavior in the ant Camponotus floridanus

Daniel F. Simola; Riley J. Graham; Cristina M. Brady; Brittany L. Enzmann; Claude Desplan; Anandasankar Ray; Laurence J. Zwiebel; Roberto Bonasio; Danny Reinberg; Juergen Liebig; Shelley L. Berger

doi:10.1126/science.aac6633

Epigenetic (re)programming of caste-specific behavior in the ant Camponotus floridanus

Daniel F. Simola, Riley J. Graham, Cristina M. Brady, Brittany L. Enzmann, Claude Desplan, Anandasankar Ray, Laurence J. Zwiebel, Roberto Bonasio, Danny Reinberg, Juergen Liebig, Shelley L. Berger

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

159 Scopus citations

Abstract

Eusocial insects organize themselves into behavioral castes whose regulation has been proposed to involve epigenetic processes, including histone modification. In the carpenter ant Camponotus floridanus, morphologically distinct worker castes called minors and majors exhibit pronounced differences in foraging and scouting behaviors. We found that these behaviors are regulated by histone acetylation likely catalyzed by the conserved acetyltransferase CBP. Transcriptome and chromatin analysis in brains of scouting minors fed pharmacological inhibitors of CBP and histone deacetylases (HDACs) revealed hundreds of genes linked to hyperacetylated regions targeted by CBP. Majors rarely forage, but injection of a HDAC inhibitor or small interfering RNAs against the HDAC Rpd3 into young major brains induced and sustained foraging in a CBP-dependent manner. Our results suggest that behavioral plasticity in animals may be regulated in an epigenetic manner via histone modification.

Original language	English (US)
Article number	aac6633
Journal	Science
Volume	351
Issue number	6268
DOIs	https://doi.org/10.1126/science.aac6633
State	Published - Jan 1 2016

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title = "Epigenetic (re)programming of caste-specific behavior in the ant Camponotus floridanus",

abstract = "Eusocial insects organize themselves into behavioral castes whose regulation has been proposed to involve epigenetic processes, including histone modification. In the carpenter ant Camponotus floridanus, morphologically distinct worker castes called minors and majors exhibit pronounced differences in foraging and scouting behaviors. We found that these behaviors are regulated by histone acetylation likely catalyzed by the conserved acetyltransferase CBP. Transcriptome and chromatin analysis in brains of scouting minors fed pharmacological inhibitors of CBP and histone deacetylases (HDACs) revealed hundreds of genes linked to hyperacetylated regions targeted by CBP. Majors rarely forage, but injection of a HDAC inhibitor or small interfering RNAs against the HDAC Rpd3 into young major brains induced and sustained foraging in a CBP-dependent manner. Our results suggest that behavioral plasticity in animals may be regulated in an epigenetic manner via histone modification.",

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AU - Simola, Daniel F.

AU - Graham, Riley J.

AU - Brady, Cristina M.

AU - Enzmann, Brittany L.

AU - Desplan, Claude

AU - Ray, Anandasankar

AU - Zwiebel, Laurence J.

AU - Bonasio, Roberto

AU - Reinberg, Danny

AU - Liebig, Juergen

AU - Berger, Shelley L.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Eusocial insects organize themselves into behavioral castes whose regulation has been proposed to involve epigenetic processes, including histone modification. In the carpenter ant Camponotus floridanus, morphologically distinct worker castes called minors and majors exhibit pronounced differences in foraging and scouting behaviors. We found that these behaviors are regulated by histone acetylation likely catalyzed by the conserved acetyltransferase CBP. Transcriptome and chromatin analysis in brains of scouting minors fed pharmacological inhibitors of CBP and histone deacetylases (HDACs) revealed hundreds of genes linked to hyperacetylated regions targeted by CBP. Majors rarely forage, but injection of a HDAC inhibitor or small interfering RNAs against the HDAC Rpd3 into young major brains induced and sustained foraging in a CBP-dependent manner. Our results suggest that behavioral plasticity in animals may be regulated in an epigenetic manner via histone modification.

AB - Eusocial insects organize themselves into behavioral castes whose regulation has been proposed to involve epigenetic processes, including histone modification. In the carpenter ant Camponotus floridanus, morphologically distinct worker castes called minors and majors exhibit pronounced differences in foraging and scouting behaviors. We found that these behaviors are regulated by histone acetylation likely catalyzed by the conserved acetyltransferase CBP. Transcriptome and chromatin analysis in brains of scouting minors fed pharmacological inhibitors of CBP and histone deacetylases (HDACs) revealed hundreds of genes linked to hyperacetylated regions targeted by CBP. Majors rarely forage, but injection of a HDAC inhibitor or small interfering RNAs against the HDAC Rpd3 into young major brains induced and sustained foraging in a CBP-dependent manner. Our results suggest that behavioral plasticity in animals may be regulated in an epigenetic manner via histone modification.

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Epigenetic (re)programming of caste-specific behavior in the ant Camponotus floridanus

Abstract

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