TY - JOUR
T1 - Epigallocatechin gallate and caffeine differentially inhibit the intestinal absorption of cholesterol and fat in ovariectomized rats
AU - Wang, Shu
AU - Noh, Sang K.
AU - Koo, Sung I.
PY - 2006/11
Y1 - 2006/11
N2 - We conducted this study to determine whether green tea constituents, (-)-epigallocatechin gallate (EGCG) and caffeine, affect the intestinal absorption of cholesterol (CH), fat, and other fat-soluble compounds. Ovariectomized rats with lymph cannula were infused intraduodenally with a lipid emulsion containing 14C-labeled CH (14C-CH), α-tocopherol (αTOH), triolein, and sodium taurocholate, without (control) or with EGCG, caffeine, or EGCG plus caffeine, in PBS, pH 6.5. The lymphatic total 14C-CH was significantly lowered by EGCG (21.1 ± 2.1% dose), caffeine (27.9 6 1.7% dose), and EGCG plus caffeine (19.3 ± 0.9% dose), compared with the control (32.4 ± 1.6% dose). The lymphatic output of esterified CH also was significantly lower in rats infused with EGCG (7.9 ± 0.7 μmol), caffeine (7.6 ± 0.2 μmol), and EGCG plus caffeine (7.5 ± 0.6 mmol) than rats in the control group (11.6 ± 1.7 μmol). Also, EGCG and caffeine significantly lowered the absorption of aTOH, another highly hydrophobic lipid. However, the lymphatic outputs of oleic acid (exogenous fatty acid marker) and other fatty acids of endogenous origin were not affected by EGCG but were markedly lowered by caffeine and EGCG plus caffeine. Caffeine significantly lowered the amount of lymph flow, regardless of whether it was infused alone (14.2 ± 3.9 mL) or with EGCG (18.6 ± 2.0 mL), compared with EGCG (22.2 ± 2.2 mL) alone and the control group (23.2 ± 3.8 mL). The caffeine-induced decline in lymph flow was associated with the lowering of lipid absorption. The results indicate that both EGCG and caffeine inhibit lipid absorption and that the inhibitory effects of the 2 tea constituents are not synergistic but mediated by distinctly different mechanisms.
AB - We conducted this study to determine whether green tea constituents, (-)-epigallocatechin gallate (EGCG) and caffeine, affect the intestinal absorption of cholesterol (CH), fat, and other fat-soluble compounds. Ovariectomized rats with lymph cannula were infused intraduodenally with a lipid emulsion containing 14C-labeled CH (14C-CH), α-tocopherol (αTOH), triolein, and sodium taurocholate, without (control) or with EGCG, caffeine, or EGCG plus caffeine, in PBS, pH 6.5. The lymphatic total 14C-CH was significantly lowered by EGCG (21.1 ± 2.1% dose), caffeine (27.9 6 1.7% dose), and EGCG plus caffeine (19.3 ± 0.9% dose), compared with the control (32.4 ± 1.6% dose). The lymphatic output of esterified CH also was significantly lower in rats infused with EGCG (7.9 ± 0.7 μmol), caffeine (7.6 ± 0.2 μmol), and EGCG plus caffeine (7.5 ± 0.6 mmol) than rats in the control group (11.6 ± 1.7 μmol). Also, EGCG and caffeine significantly lowered the absorption of aTOH, another highly hydrophobic lipid. However, the lymphatic outputs of oleic acid (exogenous fatty acid marker) and other fatty acids of endogenous origin were not affected by EGCG but were markedly lowered by caffeine and EGCG plus caffeine. Caffeine significantly lowered the amount of lymph flow, regardless of whether it was infused alone (14.2 ± 3.9 mL) or with EGCG (18.6 ± 2.0 mL), compared with EGCG (22.2 ± 2.2 mL) alone and the control group (23.2 ± 3.8 mL). The caffeine-induced decline in lymph flow was associated with the lowering of lipid absorption. The results indicate that both EGCG and caffeine inhibit lipid absorption and that the inhibitory effects of the 2 tea constituents are not synergistic but mediated by distinctly different mechanisms.
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U2 - 10.1093/jn/136.11.2791
DO - 10.1093/jn/136.11.2791
M3 - Article
C2 - 17056802
AN - SCOPUS:33751109517
SN - 0022-3166
VL - 136
SP - 2791
EP - 2796
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 11
ER -