Enzyme-assisted reforming of glucose to hydrogen in a photoelectrochemical cell

Michael Hambourger, Alicia Brune, Devens Gust, Ana Moore, Thomas Moore

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Hydrogen gas has been produced by reforming glucose in a hybrid photoelectrochemical cell that couples a dye-sensitized nanoparticulate wide band gap semiconductor photoanode to the enzyme-based oxidation of glucose. A layer of porphyrin sensitizer is adsorbed to a TiO2 nanoparticulate aggregate sintered to a conducting glass substrate to form the photoanode. Excitation of the porphyrin results in electron injection into the TiO 2, and migration to a microporous platinum cathode where hydrogen is produced by hydrogen ion reduction. The oxidized sensitizer dye is reduced by NADH, regenerating the dye and poising the NAD+/NADH redox couple oxidizing. The NAD+ is recycled to NADH by the enzyme glucose dehydrogenase, which obtains the necessary electrons from oxidation of glucose. The reforming of glucose produces gluconolactone, which hydrolyzes to gluconate; the electrochemical potential necessary to overcome thermodynamic and kinetic barriers to hydrogen production by NADH is provided by light. The quantum yield of hydrogen is ∼2.5%.

Original languageEnglish (US)
Pages (from-to)1015-1020
Number of pages6
JournalPhotochemistry and Photobiology
Volume81
Issue number4
DOIs
StatePublished - Jul 2005

Fingerprint

Photoelectrochemical cells
Reforming reactions
glucose
NAD
enzymes
Hydrogen
Glucose
hydrogen
Enzymes
cells
dyes
Coloring Agents
porphyrins
Porphyrins
oxidation
Glucose 1-Dehydrogenase
dehydrogenases
Electrons
hydrogen production
Oxidation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Enzyme-assisted reforming of glucose to hydrogen in a photoelectrochemical cell. / Hambourger, Michael; Brune, Alicia; Gust, Devens; Moore, Ana; Moore, Thomas.

In: Photochemistry and Photobiology, Vol. 81, No. 4, 07.2005, p. 1015-1020.

Research output: Contribution to journalArticle

Hambourger, Michael ; Brune, Alicia ; Gust, Devens ; Moore, Ana ; Moore, Thomas. / Enzyme-assisted reforming of glucose to hydrogen in a photoelectrochemical cell. In: Photochemistry and Photobiology. 2005 ; Vol. 81, No. 4. pp. 1015-1020.
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