Enzyme-assisted reforming of glucose to hydrogen in a photoelectrochemical cell

Michael Hambourger, Alicia Brune, Devens Gust, Ana Moore, Thomas Moore

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Abstract

Hydrogen gas has been produced by reforming glucose in a hybrid photoelectrochemical cell that couples a dye-sensitized nanoparticulate wide band gap semiconductor photoanode to the enzyme-based oxidation of glucose. A layer of porphyrin sensitizer is adsorbed to a TiO2 nanoparticulate aggregate sintered to a conducting glass substrate to form the photoanode. Excitation of the porphyrin results in electron injection into the TiO 2, and migration to a microporous platinum cathode where hydrogen is produced by hydrogen ion reduction. The oxidized sensitizer dye is reduced by NADH, regenerating the dye and poising the NAD+/NADH redox couple oxidizing. The NAD+ is recycled to NADH by the enzyme glucose dehydrogenase, which obtains the necessary electrons from oxidation of glucose. The reforming of glucose produces gluconolactone, which hydrolyzes to gluconate; the electrochemical potential necessary to overcome thermodynamic and kinetic barriers to hydrogen production by NADH is provided by light. The quantum yield of hydrogen is ∼2.5%.

Original languageEnglish (US)
Pages (from-to)1015-1020
Number of pages6
JournalPhotochemistry and Photobiology
Volume81
Issue number4
DOIs
Publication statusPublished - Jul 2005

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

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