Environmental distribution of the trichloroethene reductive dehalogenase gene (tceA) suggests lateral gene transfer among Dehalococcoides

Rosa Krajmalnik-Brown, Youlboong Sung, Kirsti M. Ritalahti, F. Michael Saunders, Frank E. Löffler

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The trichloroethene reductive dehalogenase gene (tceA) of Dehalococcoides spp. was detected in 12 of 21 trichloroethene-to-ethene dechlorinating enrichment cultures established from aquifer and sediment samples collected from diverse geographic locations in the USA. Analysis of the tceA chromosomal regions indicated that the tceA genes shared greater than 95% sequence identity, and all shared identical tceAB spacer sequences and tceB genes downstream of tceA. A putative transposable element (PTE) was present 1077 bp downstream of the tceB stop codon in three of eight chromosomal regions analyzed. Sequence identity was interrupted downstream of tceB and upstream or downstream of the PTE, suggesting that intrachromosomal or interchromosomal transfer of tceAB had occurred.

Original languageEnglish (US)
Pages (from-to)206-214
Number of pages9
JournalFEMS Microbiology Ecology
Volume59
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Horizontal Gene Transfer
gene transfer
trichloroethylene
DNA Transposable Elements
gene
Genes
Trichloroethylene
Geographic Locations
Terminator Codon
Groundwater
ethylene
aquifer
sediment
tetrachloroethene dehalogenase
distribution

Keywords

  • Chlorinated ethenes
  • Dehalococcoides
  • Lateral gene transfer
  • Reductive dechlorination
  • Reductive dehalogenase genes

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Environmental distribution of the trichloroethene reductive dehalogenase gene (tceA) suggests lateral gene transfer among Dehalococcoides. / Krajmalnik-Brown, Rosa; Sung, Youlboong; Ritalahti, Kirsti M.; Michael Saunders, F.; Löffler, Frank E.

In: FEMS Microbiology Ecology, Vol. 59, No. 1, 01.2007, p. 206-214.

Research output: Contribution to journalArticle

Krajmalnik-Brown, Rosa ; Sung, Youlboong ; Ritalahti, Kirsti M. ; Michael Saunders, F. ; Löffler, Frank E. / Environmental distribution of the trichloroethene reductive dehalogenase gene (tceA) suggests lateral gene transfer among Dehalococcoides. In: FEMS Microbiology Ecology. 2007 ; Vol. 59, No. 1. pp. 206-214.
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