TY - JOUR
T1 - Environmental distribution of the trichloroethene reductive dehalogenase gene (tceA) suggests lateral gene transfer among Dehalococcoides
AU - Krajmalnik-Brown, Rosa
AU - Sung, Youlboong
AU - Ritalahti, Kirsti M.
AU - Michael Saunders, F.
AU - Löffler, Frank E.
PY - 2007/1
Y1 - 2007/1
N2 - The trichloroethene reductive dehalogenase gene (tceA) of Dehalococcoides spp. was detected in 12 of 21 trichloroethene-to-ethene dechlorinating enrichment cultures established from aquifer and sediment samples collected from diverse geographic locations in the USA. Analysis of the tceA chromosomal regions indicated that the tceA genes shared greater than 95% sequence identity, and all shared identical tceAB spacer sequences and tceB genes downstream of tceA. A putative transposable element (PTE) was present 1077 bp downstream of the tceB stop codon in three of eight chromosomal regions analyzed. Sequence identity was interrupted downstream of tceB and upstream or downstream of the PTE, suggesting that intrachromosomal or interchromosomal transfer of tceAB had occurred.
AB - The trichloroethene reductive dehalogenase gene (tceA) of Dehalococcoides spp. was detected in 12 of 21 trichloroethene-to-ethene dechlorinating enrichment cultures established from aquifer and sediment samples collected from diverse geographic locations in the USA. Analysis of the tceA chromosomal regions indicated that the tceA genes shared greater than 95% sequence identity, and all shared identical tceAB spacer sequences and tceB genes downstream of tceA. A putative transposable element (PTE) was present 1077 bp downstream of the tceB stop codon in three of eight chromosomal regions analyzed. Sequence identity was interrupted downstream of tceB and upstream or downstream of the PTE, suggesting that intrachromosomal or interchromosomal transfer of tceAB had occurred.
KW - Chlorinated ethenes
KW - Dehalococcoides
KW - Lateral gene transfer
KW - Reductive dechlorination
KW - Reductive dehalogenase genes
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U2 - 10.1111/j.1574-6941.2006.00243.x
DO - 10.1111/j.1574-6941.2006.00243.x
M3 - Article
C2 - 17233752
AN - SCOPUS:33845645594
SN - 0168-6496
VL - 59
SP - 206
EP - 214
JO - FEMS microbiology ecology
JF - FEMS microbiology ecology
IS - 1
ER -