Enucleation of mammalian cells by cytochalasin B. I. Characterization of anucleate cells

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Abstract

Cytochalasin B, a metabolite isolated from cultures of the fungus Helminthosporium dematioideum, induced enucleation of a variety of mammalian cells in vitro when used at concentrations of 30 gmg/ml or greater. The susceptibility of different cell types to enucleation by cytochalasin varied significantly. Murine cells, notably the L929 line and peritoneal macrophages, were most susceptible to enucleation but anucleate cells were also obtained routinely from certain human and hamster cell cultures. The effect of different culture conditions on the number and viability of anucleate cells was investigated. Anucleate cells obtained by this method were viable for 24-36 h and were able to synthesize protein. The possible mechanism of cytochalasin B-induced enucleation was discussed.

Original languageEnglish (US)
Pages (from-to)273-286
Number of pages14
JournalExperimental Cell Research
Volume73
Issue number2
DOIs
StatePublished - 1972
Externally publishedYes

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Cytochalasin B
Helminthosporium
Cytochalasins
Peritoneal Macrophages
Cricetinae
Cell Survival
Fungi
Cell Culture Techniques
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Enucleation of mammalian cells by cytochalasin B. I. Characterization of anucleate cells. / Poste, George.

In: Experimental Cell Research, Vol. 73, No. 2, 1972, p. 273-286.

Research output: Contribution to journalArticle

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