Cytochalasin B, a metabolite isolated from cultures of the fungus Helminthosporium dematioideum, induced enucleation of a variety of mammalian cells in vitro when used at concentrations of 30 gmg/ml or greater. The susceptibility of different cell types to enucleation by cytochalasin varied significantly. Murine cells, notably the L929 line and peritoneal macrophages, were most susceptible to enucleation but anucleate cells were also obtained routinely from certain human and hamster cell cultures. The effect of different culture conditions on the number and viability of anucleate cells was investigated. Anucleate cells obtained by this method were viable for 24-36 h and were able to synthesize protein. The possible mechanism of cytochalasin B-induced enucleation was discussed.
ASJC Scopus subject areas
- Cell Biology