Enrichment experiment changes microbial interactions in an ultra-oligotrophic environment

Gabriel Y. Ponce-Soto, Eneas Aguirre-von-Wobeser, Luis E. Eguiarte, James Elser, Zarraz M P Lee, Valeria Souza

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The increase of nutrients in water bodies, in particular nitrogen (N) and phosphorus (P) due to the recent expansion of agricultural and other human activities is accelerating environmental degradation of these water bodies, elevating the risk of eutrophication and reducing biodiversity. To evaluate the ecological effects of the influx of nutrients in an oligotrophic and stoichiometrically imbalanced environment, we performed a replicated in situ mesocosm experiment. We analyzed the effects of a N- and P-enrichment on the bacterial interspecific interactions in an experiment conducted in the Cuatro Cienegas Basin (CCB) in Mexico. This is a desert ecosystem comprised of several aquatic systems with a large number of microbial endemic species. The abundance of key nutrients in this basin exhibits strong stoichiometric imbalance (high N:P ratios), suggesting that species diversity is maintained mostly by competition for resources. We focused on the biofilm formation and antibiotic resistance of 960 strains of cultivated bacteria in two habitats, water and sediment, before and after 3 weeks of fertilization. The water habitat was dominated by Pseudomonas, while Halomonas dominated the sediment. Strong antibiotic resistance was found among the isolates at time zero in the nutrient-poor bacterial communities, but resistance declined in the bacteria isolated in the nutrient-rich environments, suggesting that in the nutrient-poor original environment, negative inter-specific interactions were important, while in the nutrient-rich environments, competitive interactions are not so important. In water, a significant increase in the percentage of biofilm-forming strains was observed for all treatments involving nutrient addition.

Original languageEnglish (US)
Article number246
JournalFrontiers in Microbiology
Volume6
Issue numberAPR
DOIs
StatePublished - 2015

Fingerprint

Microbial Interactions
Food
Ecosystem
Body Water
Biofilms
Microbial Drug Resistance
Water
Halomonas
Eutrophication
Bacteria
Biodiversity
Pseudomonas
Mexico
Fertilization
Human Activities
Phosphorus
Nitrogen

Keywords

  • Community structure
  • Cuatro Cienegas Basin
  • Mesocosm
  • Nutrient enrichment
  • Proteobacteria interactions

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Ponce-Soto, G. Y., Aguirre-von-Wobeser, E., Eguiarte, L. E., Elser, J., Lee, Z. M. P., & Souza, V. (2015). Enrichment experiment changes microbial interactions in an ultra-oligotrophic environment. Frontiers in Microbiology, 6(APR), [246]. https://doi.org/10.3389/fmicb.2015.00246

Enrichment experiment changes microbial interactions in an ultra-oligotrophic environment. / Ponce-Soto, Gabriel Y.; Aguirre-von-Wobeser, Eneas; Eguiarte, Luis E.; Elser, James; Lee, Zarraz M P; Souza, Valeria.

In: Frontiers in Microbiology, Vol. 6, No. APR, 246, 2015.

Research output: Contribution to journalArticle

Ponce-Soto, Gabriel Y. ; Aguirre-von-Wobeser, Eneas ; Eguiarte, Luis E. ; Elser, James ; Lee, Zarraz M P ; Souza, Valeria. / Enrichment experiment changes microbial interactions in an ultra-oligotrophic environment. In: Frontiers in Microbiology. 2015 ; Vol. 6, No. APR.
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