Enhanced microcontact printing of proteins on nanoporous silica surface

Ellen Blinka, Kathryn Loeffler, Ye Hu, Ashwini Gopal, Kazunori Hoshino, Kevin Lin, Xuewu Liu, Mauro Ferrari, John X.J. Zhang

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We demonstrate porous silica surface modification, combined with microcontact printing, as an effective method for enhanced protein patterning and adsorption on arbitrary surfaces. Compared to conventional chemical treatments, this approach offers scalability and long-term device stability without requiring complex chemical activation. Two chemical surface treatments using functionalization with the commonly used 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA) were compared with the nanoporous silica surface on the basis of protein adsorption. The deposited thickness and uniformity of porous silica films were evaluated for fluorescein isothiocyanate (FITC)-labeled rabbit immunoglobulin G (R-IgG) protein printed onto the substrates via patterned polydimethlysiloxane (PDMS) stamps. A more complete transfer of proteins was observed on porous silica substrates compared to chemically functionalized substrates. A comparison of different pore sizes (4-6 nm) and porous silica thicknesses (96-200 nm) indicates that porous silica with 4 nm diameter, 57% porosity and a thickness of 96 nm provided a suitable environment for complete transfer of R-IgG proteins. Both fluorescence microscopy and atomic force microscopy (AFM) were used for protein layer characterizations. A porous silica layer is biocompatible, providing a favorable transfer medium with minimal damage to the proteins. A patterned immunoassay microchip was developed to demonstrate the retained protein function after printing on nanoporous surfaces, which enables printable and robust immunoassay detection for point-of-care applications.

Original languageEnglish (US)
Article number415302
JournalNanotechnology
Volume21
Issue number41
DOIs
StatePublished - Oct 15 2010

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Blinka, E., Loeffler, K., Hu, Y., Gopal, A., Hoshino, K., Lin, K., Liu, X., Ferrari, M., & Zhang, J. X. J. (2010). Enhanced microcontact printing of proteins on nanoporous silica surface. Nanotechnology, 21(41), [415302]. https://doi.org/10.1088/0957-4484/21/41/415302