Enhanced expression of cytokine genes in spleen macrophages during acute infection with infectious bursal disease virus in chickens

L. J. Kim, K. Karaca, T. L. Pertile, S. A. Erickson, J. M. Sharma

Research output: Contribution to journalArticle

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Abstract

We examined the effects of infectious bursal disease virus (IBDV) on splenic T cells and macrophages. In acute IBDV infection, splenocytes responded poorly to Con A stimulation. However, when T cells were isolated from whole spleen cells, purified T cells responded normally to Con A. This result indicated that functional T cells were present in the spleen but mitogen-induced proliferation of T cells was being suppressed by other cells. Previous studies indicated that soluble factors from suppressor cells may mediate this inhibition of T cell mitogenesis. We thus examined the effects of IBDV on spleen adherent cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to quantitate the expression of several cytokine genes in splenic macrophages. In acute IBDV infection, splenic macrophages exhibited enhanced gene expression of type I interferon (IFN), chicken myelomonocytic growth factor (cMGF), an avian homolog of mammalian IL-6, and 9E3/CEF4, an arian homolog of mammalian IL-8. Mitogen-stimulated spleen cell cultures also produced elevated levels of nitric oxide. The elevation of cytokine gene expression by macrophages occurred transiently during the acute phase of viral infection and coincided with in vitro inhibition of T cell mitogenic response of spleen cells.

Original languageEnglish (US)
Pages (from-to)331-341
Number of pages11
JournalVeterinary Immunology and Immunopathology
Volume61
Issue number2-4
DOIs
StatePublished - Feb 27 1998
Externally publishedYes

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Infectious bursal disease virus
Chickens
spleen
macrophages
cytokines
Spleen
T-lymphocytes
Macrophages
splenocytes
Cytokines
T-Lymphocytes
Gene Expression
Infection
infection
Virus Diseases
genes
Mitogens
suppressor cells
gene expression
Interferon Type I

Keywords

  • Chicken
  • Cytokine
  • Immunosuppression
  • Infectious bursal disease virus
  • Macrophage
  • Polymerase chain reaction (PCR)

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Enhanced expression of cytokine genes in spleen macrophages during acute infection with infectious bursal disease virus in chickens. / Kim, L. J.; Karaca, K.; Pertile, T. L.; Erickson, S. A.; Sharma, J. M.

In: Veterinary Immunology and Immunopathology, Vol. 61, No. 2-4, 27.02.1998, p. 331-341.

Research output: Contribution to journalArticle

Kim, L. J. ; Karaca, K. ; Pertile, T. L. ; Erickson, S. A. ; Sharma, J. M. / Enhanced expression of cytokine genes in spleen macrophages during acute infection with infectious bursal disease virus in chickens. In: Veterinary Immunology and Immunopathology. 1998 ; Vol. 61, No. 2-4. pp. 331-341.
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