Enhanced D-amino acid incorporation into protein by modified ribosomes

Larisa Dedkova, Nour Eddine Fahmi, Serguei Y. Golovine, Sidney Hecht

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

By overexpression of modified Escherichia coli 23S rRNAs from multicopy plasmids, ribosomes were prepared that contained mutations in two regions (2447-2450 and 2457-2462) of 23S rRNA. Following mutagenesis and selection, two clones with mutations in the 2447-2450 region (peptidyltransferase center) and six with mutations in the 2457-2462 region (helix 89) were characterized. The mutations were shown to exhibit a high level of homology. Cell-free protein synthesizing systems prepared from these clones were found to exhibit significantly enhanced incorporation of d-methionine and d-phenylalanine into protein. The incorporations involved positions 10, 22, and 54 of E. coli dihydrofolate reductase and positions 247 and 250 of Photinus pyralis firefly luciferase. Interestingly, some of the derived proteins containing the d-amino acids (notably DHFR analogues altered at position 10) functioned as well as those containing the respective l-amino acids, while substitution at other positions resulted in proteins having greatly diminished activity.

Original languageEnglish (US)
Pages (from-to)6616-6617
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number22
DOIs
StatePublished - Jun 4 2003
Externally publishedYes

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Ribosomes
Amino acids
Proteins
Amino Acids
Mutation
Escherichia coli
Clone Cells
Fireflies
Peptidyl Transferases
Firefly Luciferases
Mutagenesis
Tetrahydrofolate Dehydrogenase
Amino Acid Substitution
Phenylalanine
Methionine
Plasmids
Substitution reactions

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Enhanced D-amino acid incorporation into protein by modified ribosomes. / Dedkova, Larisa; Fahmi, Nour Eddine; Golovine, Serguei Y.; Hecht, Sidney.

In: Journal of the American Chemical Society, Vol. 125, No. 22, 04.06.2003, p. 6616-6617.

Research output: Contribution to journalArticle

Dedkova, Larisa ; Fahmi, Nour Eddine ; Golovine, Serguei Y. ; Hecht, Sidney. / Enhanced D-amino acid incorporation into protein by modified ribosomes. In: Journal of the American Chemical Society. 2003 ; Vol. 125, No. 22. pp. 6616-6617.
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