Enhanced autoreactivity of T-lymphocytes in primary sclerosing cholangitis

Keith Lindor, R. H. Wiesner, N. F. LaRusso, H. A. Homburger

Research output: Contribution to journalArticle

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Abstract

Primary sclerosing cholangitis is a chronic, cholestatic liver disease in which immune mechanisms are thought to play a role in the pathogenesis. We have determined the frequencies and functional phenotypes of autoreactive T-lymphocytes in peripheral blood specimens from patients with primary sclerosing cholangitis and healthy adults as an indicator of autoreactivity in primary sclerosing cholangitis. We found a significant increase in the percentage of autoreactive suppressor/cytotoxic T-lymphocytes that become activated in the T-, non-T-autologous mixed lymphocyte reaction in primary sclerosing cholangitis patients (p < 0.002). Blastogenesis in autologous mixed lymphocyte reaction cultures from primary sclerosing cholangitis patients was significantly decreased (p < 0.02) and the magnitude of the decrease correlated directly with the percentage of activated suppressor/cytotoxic T-lymphocytes (r = 0.56, p < 0.01). The percentage of autoreactive, suppressor T-lymphocytes was increased in autologous mixed lymphocyte reaction cultures from primary sclerosing cholangitis patients (p < 0.0001); and suppression of mitogen-induced blastogenesis by autologous concanavalin A-treated mononuclear cells was significantly enhanced. These results, which were unrelated to the histologic stage of liver disease and the presence or absence of active colitis, document the presence of an expanded population of T-lymphocytes in the peripheral blood of patients with primary sclerosing cholangitis that became activated on exposure to autologous major histocompatibility antigens in the autologous mixed lymphocyte reaction. We hypothesize that these autoreactive cells may be a marker or may participate as effector cells in cell-mediated autoimmunity in primary sclerosing cholangitis.

Original languageEnglish (US)
Pages (from-to)884-888
Number of pages5
JournalHepatology
Volume7
Issue number5
StatePublished - 1987
Externally publishedYes

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Sclerosing Cholangitis
T-Lymphocytes
Mixed Lymphocyte Culture Test
Cytotoxic T-Lymphocytes
Lymphocyte Activation
Liver Diseases
Histocompatibility Antigens
Colitis
Concanavalin A
Autoimmunity
Mitogens
Phenotype

ASJC Scopus subject areas

  • Hepatology

Cite this

Lindor, K., Wiesner, R. H., LaRusso, N. F., & Homburger, H. A. (1987). Enhanced autoreactivity of T-lymphocytes in primary sclerosing cholangitis. Hepatology, 7(5), 884-888.

Enhanced autoreactivity of T-lymphocytes in primary sclerosing cholangitis. / Lindor, Keith; Wiesner, R. H.; LaRusso, N. F.; Homburger, H. A.

In: Hepatology, Vol. 7, No. 5, 1987, p. 884-888.

Research output: Contribution to journalArticle

Lindor, K, Wiesner, RH, LaRusso, NF & Homburger, HA 1987, 'Enhanced autoreactivity of T-lymphocytes in primary sclerosing cholangitis', Hepatology, vol. 7, no. 5, pp. 884-888.
Lindor K, Wiesner RH, LaRusso NF, Homburger HA. Enhanced autoreactivity of T-lymphocytes in primary sclerosing cholangitis. Hepatology. 1987;7(5):884-888.
Lindor, Keith ; Wiesner, R. H. ; LaRusso, N. F. ; Homburger, H. A. / Enhanced autoreactivity of T-lymphocytes in primary sclerosing cholangitis. In: Hepatology. 1987 ; Vol. 7, No. 5. pp. 884-888.
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