Engineering Escherichia coli for renewable benzyl alcohol production

Shawn Pugh, Rebekah McKenna, Ibrahim Halloum, David Nielsen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (. S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1. mg/L were realized, representing a yield of 7.6±0.1. mg/g on glucose and a ~5-fold improvement over initial strains.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalMetabolic Engineering Communications
Volume2
DOIs
StatePublished - Dec 1 2015

Fingerprint

Benzyl Alcohol
Escherichia coli
Alcohols
Biosynthesis
Glucose
Aromatic Hydrocarbons
Aromatic Amino Acids
Pseudomonas putida
Carboxy-Lyases
Alcohol Dehydrogenase
Fragrances
Cosmetics
Flavors
Aromatic hydrocarbons
Industry
Carboxylic acids
Drug products
Amino acids
Availability
Pharmaceutical Preparations

Keywords

  • Aromatic chemicals
  • Benzaldehyde
  • Benzyl alcohol

ASJC Scopus subject areas

  • Biomedical Engineering
  • Endocrinology, Diabetes and Metabolism

Cite this

Engineering Escherichia coli for renewable benzyl alcohol production. / Pugh, Shawn; McKenna, Rebekah; Halloum, Ibrahim; Nielsen, David.

In: Metabolic Engineering Communications, Vol. 2, 01.12.2015, p. 39-45.

Research output: Contribution to journalArticle

Pugh, Shawn ; McKenna, Rebekah ; Halloum, Ibrahim ; Nielsen, David. / Engineering Escherichia coli for renewable benzyl alcohol production. In: Metabolic Engineering Communications. 2015 ; Vol. 2. pp. 39-45.
@article{9b0a9a304f524792a3110a7ce3a2055f,
title = "Engineering Escherichia coli for renewable benzyl alcohol production",
abstract = "Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (. S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1. mg/L were realized, representing a yield of 7.6±0.1. mg/g on glucose and a ~5-fold improvement over initial strains.",
keywords = "Aromatic chemicals, Benzaldehyde, Benzyl alcohol",
author = "Shawn Pugh and Rebekah McKenna and Ibrahim Halloum and David Nielsen",
year = "2015",
month = "12",
day = "1",
doi = "10.1016/j.meteno.2015.06.002",
language = "English (US)",
volume = "2",
pages = "39--45",
journal = "Metabolic Engineering Communications",
issn = "2214-0301",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Engineering Escherichia coli for renewable benzyl alcohol production

AU - Pugh, Shawn

AU - McKenna, Rebekah

AU - Halloum, Ibrahim

AU - Nielsen, David

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (. S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1. mg/L were realized, representing a yield of 7.6±0.1. mg/g on glucose and a ~5-fold improvement over initial strains.

AB - Benzyl alcohol is an aromatic hydrocarbon used as a solvent and an intermediate chemical in the pharmaceutical, cosmetics, and flavor/fragrance industries. The de novo biosynthesis of benzyl alcohol directly from renewable glucose was herein explored using a non-natural pathway engineered in Escherichia coli. Benzaldehyde was first produced from endogenous phenylpyruvate via three heterologous steps, including hydroxymandelate synthase (encoded by hmaS) from Amycolatopsis orientalis, followed by (. S)-mandelate dehydrogenase (encoded by mdlB) and phenylglyoxylate decarboxylase (encoded by mdlC) from Pseudomonas putida ATCC 12633. The subsequent rapid and efficient reduction of benzaldehyde to benzyl alcohol occurred by the combined activity and native regulation of multiple endogenous alcohol dehydrogenases and/or aldo-keto reductases. Through systematic deletion of competing aromatic amino acid biosynthesis pathways to promote endogenous phenylpyruvate availability, final benzyl alcohol titers as high as 114±1. mg/L were realized, representing a yield of 7.6±0.1. mg/g on glucose and a ~5-fold improvement over initial strains.

KW - Aromatic chemicals

KW - Benzaldehyde

KW - Benzyl alcohol

UR - http://www.scopus.com/inward/record.url?scp=84933074087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84933074087&partnerID=8YFLogxK

U2 - 10.1016/j.meteno.2015.06.002

DO - 10.1016/j.meteno.2015.06.002

M3 - Article

VL - 2

SP - 39

EP - 45

JO - Metabolic Engineering Communications

JF - Metabolic Engineering Communications

SN - 2214-0301

ER -