Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells

Chien Wen Chang, Yongsung Hwang, David Brafman, Thomas Hagan, Catherine Phung, Shyni Varghese

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Cost-effective and scalable synthetic matrices that support long-term expansion of human pluripotent stem cells (hPSCs) have many applications, ranging from drug screening platforms to regenerative medicine. Here, we report the development of a hydrogel-based matrix containing synthetic heparin-mimicking moieties that supports the long-term expansion of hPSCs (≥20 passages) in a chemically defined medium. HPSCs expanded on this synthetic matrix maintained their characteristic morphology, colony forming ability, karyotypic stability, and differentiation potential. We also used the synthetic matrix as a platform to investigate the effects of various physicochemical properties of the extracellular environment on the adhesion, growth, and self-renewal of hPSCs. The observed cellular responses can be explained in terms of matrix interface-mediated binding of extracellular matrix proteins, growth factors, and other cell-secreted factors, which create an instructive microenvironment to support self-renewal of hPSCs. These synthetic matrices, which comprise of " off-the-shelf" components and are easy to synthesize, provide an ideal tool to elucidate the molecular mechanisms that control stem cell fate.

Original languageEnglish (US)
Pages (from-to)912-921
Number of pages10
JournalBiomaterials
Volume34
Issue number4
DOIs
StatePublished - Jan 2013
Externally publishedYes

Fingerprint

Cell engineering
Cell Engineering
Pluripotent Stem Cells
Stem cells
Preclinical Drug Evaluations
Regenerative Medicine
Extracellular Matrix Proteins
Hydrogel
Heparin
Intercellular Signaling Peptides and Proteins
Stem Cells
Hydrogels
Screening
Costs and Cost Analysis
Adhesion
Proteins
Growth
Pharmaceutical Preparations
Costs

Keywords

  • Embryonic stem cells
  • Human pluripotent stem cells
  • Physicochemical cues
  • Self-renewal
  • Synthetic heparin mimics
  • Synthetic matrices

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells. / Chang, Chien Wen; Hwang, Yongsung; Brafman, David; Hagan, Thomas; Phung, Catherine; Varghese, Shyni.

In: Biomaterials, Vol. 34, No. 4, 01.2013, p. 912-921.

Research output: Contribution to journalArticle

Chang, Chien Wen ; Hwang, Yongsung ; Brafman, David ; Hagan, Thomas ; Phung, Catherine ; Varghese, Shyni. / Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells. In: Biomaterials. 2013 ; Vol. 34, No. 4. pp. 912-921.
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