Engineering an Enzyme for Direct Electrical Monitoring of Activity

Bintian Zhang, Hanqing Deng, Sohini Mukherjee, Weisi Song, Xu Wang, Stuart Lindsay

Research output: Contribution to journalArticle

Abstract

Proteins have been shown to be electrically conductive if tethered to an electrode by means of a specific binding agent, allowing single molecules to be wired into an electrical sensing circuit. Such circuits allow enzymes to be used as sensors, detectors, and sequencing devices. We have engineered contact points into a φ29 polymerase by introducing biotinylatable peptide sequences. The modified enzyme was bound to electrodes functionalized with streptavidin. φ29 connected by one biotinylated contact, and a second nonspecific contact showed rapid small fluctuations in current when activated. Signals were greatly enhanced with two specific contacts. Features in the distributions of DC conductance increased by a factor 2 or more over the open to closed conformational transition of the polymerase. Polymerase activity is manifested by a rapid (millisecond) large (25% of background) current fluctuations imposed on the DC conductance.

Original languageEnglish (US)
JournalACS nano
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

enzymes
Enzymes
direct current
engineering
Electrodes
sequencing
Streptavidin
electrodes
Networks (circuits)
Monitoring
Point contacts
Peptides
peptides
Detectors
proteins
Proteins
Molecules
sensors
detectors
Sensors

Keywords

  • bioelectronic circuits
  • bioelectronics
  • polymerase activity
  • protein conductivity
  • single molecule conductance

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Engineering an Enzyme for Direct Electrical Monitoring of Activity. / Zhang, Bintian; Deng, Hanqing; Mukherjee, Sohini; Song, Weisi; Wang, Xu; Lindsay, Stuart.

In: ACS nano, 01.01.2019.

Research output: Contribution to journalArticle

Zhang, Bintian ; Deng, Hanqing ; Mukherjee, Sohini ; Song, Weisi ; Wang, Xu ; Lindsay, Stuart. / Engineering an Enzyme for Direct Electrical Monitoring of Activity. In: ACS nano. 2019.
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