@article{79d8462a558541cfb54e37e72c1ba2e3,
title = "Energy Transfer Induced by Dye Encapsulation in a Hybrid Nanoparticle-Purple Membrane Reversible Assembly",
abstract = "The purple membrane (PM) isolated from the bacteria Halobacterium salinarum (H. salinarum) arranges the transmembrane proton pump bacteriorhodopsin (bR) in a 2D hexagonal crystalline lattice. Here, PM sheets containing native bR bend into tube-like structures with open edges under acidic pH conditions. When decorated with gold nanoparticles (AuNPs), these same PM sheets yield a sealed tube assembly. Upon Rhodamine B (Rh B) sequestration inside the sealed tube, a dramatic decrease in Rh B fluorescence lifetime (τf) from 1.5 ns (unencapsulated) to 14 ps (encapsulated) is observed. The dramatic decrease in lifetime is attributed to energy transfer between AuNPs and Rh B. Subsequent release from the AuNP–PM capsules triggered by an increase in pH shows that 93% of Rh B is recovered (τ = 14 min) due to the capsules' unfolding. The hybrid AuNP–PM material highlights the utility of multicomponent ensembles (i.e., lipid bilayer, protein array, and NPs) by demonstrating that complex, multistimuli 3D responses can lead to multiplexed functions such as controlling energy transfer in the confined, encapsulated state and breaking the energy pair via molecular release in response to a change in pH.",
keywords = "bacteriorhodopsin, controlled release system, energy transfer, gold nanoparticles, nano–bio hybrid assembly, purple membrane",
author = "{de Q. Silveira}, Gleiciani and Zhaowei Chen and Barry, {Edward F.} and Diroll, {Benjamin T.} and Byeongdu Lee and Tijana Rajh and Rozhkova, {Elena A.} and Laible, {Philip D.} and Fry, {H. Christopher}",
note = "Funding Information: G.d.Q.S. and Z.C. contributed equally to this work. This material is based upon work supported by Laboratory Directed Research and Development (LDRD) and use of the Center for Nanoscale Materials was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under contract no. DE-AC02- 06CH11357. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357 for SAXS and WAXS measurement. The authors thank Gregory Tira from Biosciences Division at Argonne National Laboratory for production of PM. Funding Information: G.d.Q.S. and Z.C. contributed equally to this work. This material is based upon work supported by Laboratory Directed Research and Development (LDRD) and use of the Center for Nanoscale Materials was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under contract no. DE‐AC02‐ 06CH11357. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE‐AC02‐06CH11357 for SAXS and WAXS measurement. The authors thank Gregory Tira from Biosciences Division at Argonne National Laboratory for production of PM. Publisher Copyright: {\textcopyright} 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2019",
month = oct,
day = "1",
doi = "10.1002/adfm.201904899",
language = "English (US)",
volume = "29",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "Wiley-VCH Verlag",
number = "43",
}