Abstract

Nitrous oxide (N 2 O) is a highly energetic oxidant. This work documents a novel process that recovers N 2 O by inhibiting the activity of the nitrous-oxide reductase (N 2 OR) in denitrifying bacteria. N 2 O recovery was demonstrated in batch experiments in which NO was the only electron-acceptor, with Fe(II)EDTA being the chelating agent for NO. Increasing NO-Fe(II)EDTA led to greater accumulation of N 2 O over N 2 in batch studies (up to 70% of total gas produced), and N 2 O accumulation correlated to loss of nitrous-oxide reductase (N 2 OR) activity and conformational changes to cytochrome c, the immediate electron donor for N 2 OR. Stable and high production of N 2 O was also documented in long-term experiments with NO-Fe(II)EDTA as the only acceptor for denitrifying biomass. Mechanistically, the conformational change of cytochrome c was the dominant cause for the loss of electron-transfer capacity, which led to a decrease of N 2 OR activity and the accumulation of N 2 O. And this is the first time to recover energy source from flue gas in the form of N 2 O. Recovery of N 2 O by suppressing N 2 OR widens the options for energy recovery from nitrogen-based wastes.

Original languageEnglish (US)
Pages (from-to)500-506
Number of pages7
JournalChemical Engineering Journal
Volume371
DOIs
StatePublished - Sep 1 2019

Fingerprint

Ethylenediaminetetraacetic acid
nitrous oxide
EDTA
Bacteria
Cytochromes c
Recovery
electron
bacterium
Oxides
cytochrome
Electrons
Proteins
chelating agent
Nitrous Oxide
Chelating Agents
Chelation
Oxidants
Flue gases
oxidant
Biomass

Keywords

  • Conformational change
  • Denitrification
  • N O recovery
  • N OR inhibition

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Energy recovery in the form of N 2 O by denitrifying bacteria . / Yu, Cong; Qiao, Sen; Yang, Yue; Jin, Ruofei; Zhou, Jiti; Rittmann, Bruce.

In: Chemical Engineering Journal, Vol. 371, 01.09.2019, p. 500-506.

Research output: Contribution to journalArticle

Yu, Cong ; Qiao, Sen ; Yang, Yue ; Jin, Ruofei ; Zhou, Jiti ; Rittmann, Bruce. / Energy recovery in the form of N 2 O by denitrifying bacteria In: Chemical Engineering Journal. 2019 ; Vol. 371. pp. 500-506.
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