Endoribonuclease activity associated with animal RNA viruses

D. Kolakofsky, Sidney Altman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A specific endoribonucleolytic activity was detected when detergent-lysed vesicular stomatitis or Sendai virus was incubated with the precursor to Escherichia coli tRNA(Tyr). The cleavage products produced and the characteristics of the reaction were similar to those previously reported for human KB cell RNase NU. Like RNase NU, the virus-associated reaction generates 5'-hydroxyl and 3'-phosphate groups at the cleavage sites. At protein concentrations similar to those used to test vesicular stomatitis and Sendai viruses, virions of Sindbis virus and poliovirus also exhibited endoribonucleolytic activity, but reovirus, simian virus 40, and minute virus of mice did not. This endoribonuclease may be of physiological relevance to some of the viruses the authors tested.

Original languageEnglish (US)
Pages (from-to)274-284
Number of pages11
JournalJournal of Virology
Volume25
Issue number1
StatePublished - 1978
Externally publishedYes

Fingerprint

endoribonucleases
Endoribonucleases
Sendai virus
Vesiculovirus
Vesicular Stomatitis
RNA Viruses
Ribonucleases
Minute Virus of Mice
RNA, Transfer, Tyr
Sindbis virus
Simian virus 40
Sindbis Virus
Viruses
KB Cells
Enterovirus C
viruses
Reoviridae
Poliovirus
virion
detergents

ASJC Scopus subject areas

  • Immunology

Cite this

Endoribonuclease activity associated with animal RNA viruses. / Kolakofsky, D.; Altman, Sidney.

In: Journal of Virology, Vol. 25, No. 1, 1978, p. 274-284.

Research output: Contribution to journalArticle

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