Empirical evaluation of preservation methods for faecal DNA

M. A J Frantzen, Joan Silk, J. W H Ferguson, R. K. Wayne, M. H. Kohn

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at -20 °C and drying performed approximately equally well for mitochondrial DNA and short (< 200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.

Original languageEnglish (US)
Pages (from-to)1423-1428
Number of pages6
JournalMolecular Ecology
Volume7
Issue number10
DOIs
StatePublished - Oct 1998
Externally publishedYes

Fingerprint

nuclear genome
DNA
mitochondrial DNA
feces
Mitochondrial DNA
Feces
Papio
DEET
Polymerase Chain Reaction
genotyping
freezing
Dimethyl Sulfoxide
drying
methodology
EDTA
ethanol
Edetic Acid
Freezing
salts
Amplification

Keywords

  • Apolipoprotein E
  • Cytochrome c oxidase II
  • DNA preservation
  • Molecular scatology
  • Noninvasive sampling
  • Papio cynocephalus ursinus

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Frantzen, M. A. J., Silk, J., Ferguson, J. W. H., Wayne, R. K., & Kohn, M. H. (1998). Empirical evaluation of preservation methods for faecal DNA. Molecular Ecology, 7(10), 1423-1428. https://doi.org/10.1046/j.1365-294x.1998.00449.x

Empirical evaluation of preservation methods for faecal DNA. / Frantzen, M. A J; Silk, Joan; Ferguson, J. W H; Wayne, R. K.; Kohn, M. H.

In: Molecular Ecology, Vol. 7, No. 10, 10.1998, p. 1423-1428.

Research output: Contribution to journalArticle

Frantzen, MAJ, Silk, J, Ferguson, JWH, Wayne, RK & Kohn, MH 1998, 'Empirical evaluation of preservation methods for faecal DNA', Molecular Ecology, vol. 7, no. 10, pp. 1423-1428. https://doi.org/10.1046/j.1365-294x.1998.00449.x
Frantzen, M. A J ; Silk, Joan ; Ferguson, J. W H ; Wayne, R. K. ; Kohn, M. H. / Empirical evaluation of preservation methods for faecal DNA. In: Molecular Ecology. 1998 ; Vol. 7, No. 10. pp. 1423-1428.
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