Abstract
We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at -20 °C and drying performed approximately equally well for mitochondrial DNA and short (< 200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1423-1428 |
| Number of pages | 6 |
| Journal | Molecular Ecology |
| Volume | 7 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1998 |
| Externally published | Yes |
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Keywords
- Apolipoprotein E
- Cytochrome c oxidase II
- DNA preservation
- Molecular scatology
- Noninvasive sampling
- Papio cynocephalus ursinus
ASJC Scopus subject areas
- Ecology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
Empirical evaluation of preservation methods for faecal DNA. / Frantzen, M. A J; Silk, Joan; Ferguson, J. W H; Wayne, R. K.; Kohn, M. H.
In: Molecular Ecology, Vol. 7, No. 10, 10.1998, p. 1423-1428.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Empirical evaluation of preservation methods for faecal DNA
AU - Frantzen, M. A J
AU - Silk, Joan
AU - Ferguson, J. W H
AU - Wayne, R. K.
AU - Kohn, M. H.
PY - 1998/10
Y1 - 1998/10
N2 - We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at -20 °C and drying performed approximately equally well for mitochondrial DNA and short (< 200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.
AB - We evaluate the relative effectiveness of four methods for preserving faecal samples for DNA analysis. PCR assays of fresh faecal samples collected from free-ranging baboons showed that amplification success was dependent on preservation method, PCR-product size, and whether nuclear or mitochondrial DNA was assayed. Storage in a DMSO/EDTA/Tris/salt solution (DETs) was most effective for preserving nuclear DNA, but storage in 70% ethanol, freezing at -20 °C and drying performed approximately equally well for mitochondrial DNA and short (< 200 bp) nuclear DNA fragments. Because faecal DNA is diluted and degraded, repeated extractions from faeces may be necessary and short nuclear markers should be employed for genotyping. A review of molecular scatology studies further suggests that three to six faeces per individual should be collected.
KW - Apolipoprotein E
KW - Cytochrome c oxidase II
KW - DNA preservation
KW - Molecular scatology
KW - Noninvasive sampling
KW - Papio cynocephalus ursinus
UR - http://www.scopus.com/inward/record.url?scp=0032188995&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032188995&partnerID=8YFLogxK
U2 - 10.1046/j.1365-294x.1998.00449.x
DO - 10.1046/j.1365-294x.1998.00449.x
M3 - Article
VL - 7
SP - 1423
EP - 1428
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 10
ER -