TY - JOUR
T1 - Emerging investigator series
T2 - a multispecies analysis of the relationship between oxygen content and toxicity in graphene oxide
AU - Barrios, Ana C.
AU - Cahue, Yaritza P.
AU - Wang, Yan
AU - Geiger, Jason
AU - Puerari, Rodrigo C.
AU - Matias, William Gerson
AU - Melegari, Silvia Pedroso
AU - Gilbertson, Leanne M.
AU - Perreault, François
N1 - Publisher Copyright:
© The Royal Society of Chemistry 2021.
PY - 2021/6
Y1 - 2021/6
N2 - The toxicity of graphene oxide (GO) has been documented for multiple species. However, GO has variable surface chemistry, and it is currently unclear whether changes in oxygen content impact GO-organism interactions the same way across species. In this study, a modified Hummer's GO (ARGO) was systematically reduced by thermal annealing at 200, 500, or 800 °C and toxicity towards bacteria (Escherichia coli), alga (Scenedesmus obliquus), cyanobacteria (Microcystis aeruginosa), and invertebrates (Daphnia magna) was assessed by measuring the effective concentrations inducing 50% inhibition (EC50). The EC50-carbon/oxygen ratio relationships show similar trends for bacteria and invertebrates, where toxicity increases as the material is reduced. Conversely, cyanobacterial inhibition decreases as GO is reduced. Further testing supports differences in cell-GO interactions between bacteria and cyanobacteria. Cyanobacteria showed a decrease in metabolic activity, evidenced by a 69% reduction in esterase activity after ARGO exposure but no oxidative stress, measured by 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescence and catalase activity. In contrast, ARGO induced a 55% increase in H2DCFDA fluorescence and 342% increase in catalase activity in bacteria. These changes in cell-material interactions propose different mechanisms of action, a physical mechanism occurring in cyanobacteria, and a chemical mechanism in bacteria. The differences in GO toxicity observed in different organisms emphasize the need to differentiate the safe-by-design guidelines made for GO in relation to the potential organisms exposed.
AB - The toxicity of graphene oxide (GO) has been documented for multiple species. However, GO has variable surface chemistry, and it is currently unclear whether changes in oxygen content impact GO-organism interactions the same way across species. In this study, a modified Hummer's GO (ARGO) was systematically reduced by thermal annealing at 200, 500, or 800 °C and toxicity towards bacteria (Escherichia coli), alga (Scenedesmus obliquus), cyanobacteria (Microcystis aeruginosa), and invertebrates (Daphnia magna) was assessed by measuring the effective concentrations inducing 50% inhibition (EC50). The EC50-carbon/oxygen ratio relationships show similar trends for bacteria and invertebrates, where toxicity increases as the material is reduced. Conversely, cyanobacterial inhibition decreases as GO is reduced. Further testing supports differences in cell-GO interactions between bacteria and cyanobacteria. Cyanobacteria showed a decrease in metabolic activity, evidenced by a 69% reduction in esterase activity after ARGO exposure but no oxidative stress, measured by 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescence and catalase activity. In contrast, ARGO induced a 55% increase in H2DCFDA fluorescence and 342% increase in catalase activity in bacteria. These changes in cell-material interactions propose different mechanisms of action, a physical mechanism occurring in cyanobacteria, and a chemical mechanism in bacteria. The differences in GO toxicity observed in different organisms emphasize the need to differentiate the safe-by-design guidelines made for GO in relation to the potential organisms exposed.
UR - http://www.scopus.com/inward/record.url?scp=85108165900&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85108165900&partnerID=8YFLogxK
U2 - 10.1039/d0en01264e
DO - 10.1039/d0en01264e
M3 - Article
AN - SCOPUS:85108165900
SN - 2051-8153
VL - 8
SP - 1543
EP - 1559
JO - Environmental Science: Nano
JF - Environmental Science: Nano
IS - 6
ER -