Emergent properties of nanosensor arrays: Applications for monitoring IgG affinity distributions, weakly affined hypermannosylation, and colony selection for biomanufacturing

Nigel F. Reuel, Brittany Grassbaugh, Sebastian Kruss, J. Zachary Mundy, Cary Opel, Adebola O. Ogunniyi, Kamal Egodage, Ramon Wahl, Bernhard Helk, Jingqing Zhang, Z. Ilke Kalcioglu, Kevin Tvrdy, Darin O. Bellisario, Bin Mu, Steven S. Blake, Krystyn J. Van Vliet, J. Christopher Love, Karl Dane Wittrup, Michael S. Strano

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

It is widely recognized that an array of addressable sensors can be multiplexed for the label-free detection of a library of analytes. However, such arrays have useful properties that emerge from the ensemble, even when monofunctionalized. As examples, we show that an array of nanosensors can estimate the mean and variance of the observed dissociation constant (K D), using three different examples of binding IgG with Protein A as the recognition site, including polyclonal human IgG (KD μ = 19 μM, σ2 = 1000 mM2), murine IgG (KD μ = 4.3 nM, σ2 = 3 μM2), and human IgG from CHO cells (KD μ = 2.5 nM, σ2 = 0.01 μM 2). Second, we show that an array of nanosensors can uniquely monitor weakly affined analyte interactions via the increased number of observed interactions. One application involves monitoring the metabolically induced hypermannosylation of human IgG from CHO using PSA-lectin conjugated sensor arrays where temporal glycosylation patterns are measured and compared. Finally, the array of sensors can also spatially map the local production of an analyte from cellular biosynthesis. As an example, we rank productivity of IgG-producing HEK colonies cultured directly on the array of nanosensors itself.

Original languageEnglish (US)
Pages (from-to)7472-7482
Number of pages11
JournalACS nano
Volume7
Issue number9
DOIs
StatePublished - Sep 24 2013

Keywords

  • affinity distribution
  • array
  • biomanufacturing
  • glycan
  • nanotube
  • sensor

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy

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