Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferons as adjuvants

Silke Rautenschlein, Jagdev M. Sharma, Barbara J. Winslow, Janis McMillen, David Junker, Mark Cochran

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Recombinant fowlpox viruses (rFPV) expressing the fusion and hemagglutinin-neuraminidase glycoproteins of Newcastle disease virus (NDV) as well as chicken type I interferon (IFN) or type II IFN were used to vaccinate specific pathogen-free (SPF) turkeys in ovo. No significant changes in the hatchability, survival rate, performance and weight gain were observed after vaccination with the rFPV vaccines in comparison to diluent-inoculated embryos. The rFPV-NDV-IFN-II construct induced the onset of anti-NDV antibody production in SPF birds at one week post hatch, one week earlier than other vaccine constructs. Three to five weeks post hatch, the turkeys were challenged with the neurotropic velogenic NDV strain Texas GB (NDV-GB-Tx). The rFPV-NDV-IFN-II construct was the most protective vaccine against NDV. rFPV vaccines significantly (p<0.05) suppressed the mitogenic response of peripheral blood leukocytes in vaccinated turkeys in comparison to placebo inoculated controls at 25 days post vaccination. Birds vaccinated with rFPV-NDV-IFN-I construct did not have an inhibition in the mitogenic response. Copyright (C) 1999 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)426-433
Number of pages8
JournalVaccine
Volume18
Issue number5-6
DOIs
StatePublished - Oct 14 1999

Keywords

  • In ovo
  • Interferons
  • Turkeys

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferons as adjuvants'. Together they form a unique fingerprint.

  • Cite this