Abstract

Filtration for microfluidic sample-collection devices is desirable for sample selection, concentration, preprocessing, and manipulation, but microfabricating the required sub-micrometer structures is an elaborate process. This article presents a simple method to integrate filters in polydimethylsiloxane (PDMS) devices to sample microorganisms in aqueous environments. An off-the-shelf membrane filter with 0.22-μm pores was embedded in a PDMS layer and sequentially bound with other PDMS channel layers. No leakage was observed during filtration. This device was validated by concentrating a large amount of biomass, from 15 × 10 7 to 3 × 10 8 cells/ml of cyanobacterium Synechocystis in simulated sample water with consistent performance across devices. The major advantagesof this method are low cost, simple design, straightforward fabrication, and robust performance, enabling wide-utility of chip-based devices for field-deployable operations in environmental microbiology.

Original languageEnglish (US)
Pages (from-to)221-225
Number of pages5
JournalMicrofluidics and Nanofluidics
Volume11
Issue number2
DOIs
StatePublished - Aug 2011

Fingerprint

microorganisms
Polydimethylsiloxane
shelves
embedding
sampling
chips
Sampling
filters
Environmental microbiology
Microfluidics
Microorganisms
microbiology
Biomass
Membranes
concentrating
Fabrication
preprocessing
biomass
Water
micrometers

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Cite this

Embedding off-the-shelf filter in PDMS chip for microbe sampling. / Lécluse, Aurélie; Chao, Shih-Hui; Meldrum, Deirdre.

In: Microfluidics and Nanofluidics, Vol. 11, No. 2, 08.2011, p. 221-225.

Research output: Contribution to journalArticle

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