Elongational-flow-induced scission of DNA nanotubes in laminar flow

Rizal Hariadi, Bernard Yurke

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The length distributions of polymer fragments subjected to an elongational-flow-induced scission are profoundly affected by the fluid flow and the polymer bond strengths. In this paper, laminar elongational flow was used to induce chain scission of a series of circumference-programmed DNA nanotubes. The DNA nanotubes served as a model system for semiflexible polymers with tunable bond strength and cross-sectional geometry. The expected length distribution of fragmented DNA nanotubes was calculated from first principles by modeling the interplay between continuum hydrodynamic elongational flow and the molecular forces required to overstretch multiple DNA double helices. Our model has no-free parameters; the only inferred parameter is obtained from DNA mechanics literature, namely, the critical tension required to break a DNA duplex into two single-stranded DNA strands via the overstretching B-S DNA transition. The nanotube fragments were assayed with fluorescence microscopy at the single-molecule level and their lengths are in agreement with the scission theory.

Original languageEnglish (US)
Article number046307
JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume82
Issue number4
DOIs
StatePublished - Oct 19 2010
Externally publishedYes

Fingerprint

Laminar Flow
laminar flow
Nanotubes
cleavage
nanotubes
deoxyribonucleic acid
Polymers
Fragment
Expected Length
Fluorescence Microscopy
Circumference
First-principles
Helix
Fluid Flow
Mechanics
Hydrodynamics
polymers
Continuum
fragments
Series

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Statistical and Nonlinear Physics
  • Statistics and Probability

Cite this

Elongational-flow-induced scission of DNA nanotubes in laminar flow. / Hariadi, Rizal; Yurke, Bernard.

In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 82, No. 4, 046307, 19.10.2010.

Research output: Contribution to journalArticle

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