Electrophoretic exclusion for the selective transport of small molecules

Michelle M. Meighan, Michael W. Keebaugh, Alicia M. Quihuis, Stacy M. Kenyon, Mark Hayes

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A novel method capable of differentiating and concentrating small molecules in bulk solution termed "electrophoretic exclusion" is described and experimentally investigated. In this technique, the hydrodynamic flow of the system is countered by the electrophoretic velocity to prevent a species from entering into the channel. The separation can be controlled by changing the flow rate or applied electric field in order to exclude certain species selectively while allowing others to pass through the capillary. Proof of principle studies employed a flow injection regime of the method and examined the exclusion of Methyl Violet dye in the presence of a neutral species. Methyl Violet was concentrated almost 40 times the background concentration in 30 s using 6 kV. Additionally, a threshold voltage necessary for exclusion was determined. The establishment of a threshold voltage enabled the differentiation of two similar cationic species: Methyl Green and Neutral Red.

Original languageEnglish (US)
Pages (from-to)3786-3792
Number of pages7
JournalElectrophoresis
Volume30
Issue number21
DOIs
StatePublished - 2009

Fingerprint

Gentian Violet
Threshold voltage
Methyl Green
Molecules
Hydrodynamics
Coloring Agents
Electric fields
Flow rate
Injections

Keywords

  • Counterflow
  • Electrocapture
  • Electrophoretic exclusion
  • Gradient separations

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Meighan, M. M., Keebaugh, M. W., Quihuis, A. M., Kenyon, S. M., & Hayes, M. (2009). Electrophoretic exclusion for the selective transport of small molecules. Electrophoresis, 30(21), 3786-3792. https://doi.org/10.1002/elps.200900340

Electrophoretic exclusion for the selective transport of small molecules. / Meighan, Michelle M.; Keebaugh, Michael W.; Quihuis, Alicia M.; Kenyon, Stacy M.; Hayes, Mark.

In: Electrophoresis, Vol. 30, No. 21, 2009, p. 3786-3792.

Research output: Contribution to journalArticle

Meighan, MM, Keebaugh, MW, Quihuis, AM, Kenyon, SM & Hayes, M 2009, 'Electrophoretic exclusion for the selective transport of small molecules', Electrophoresis, vol. 30, no. 21, pp. 3786-3792. https://doi.org/10.1002/elps.200900340
Meighan, Michelle M. ; Keebaugh, Michael W. ; Quihuis, Alicia M. ; Kenyon, Stacy M. ; Hayes, Mark. / Electrophoretic exclusion for the selective transport of small molecules. In: Electrophoresis. 2009 ; Vol. 30, No. 21. pp. 3786-3792.
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