Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse

L. Galli, S. K. Son, M. Klinge, S. Bajt, A. Barty, R. Bean, C. Betzel, K. R. Beyerlein, C. Caleman, R. B. Doak, M. Duszenko, H. Fleckenstein, C. Gati, B. Hunt, Richard Kirian, M. Liang, M. H. Nanao, K. Nass, D. Oberthür, L. Redecke & 7 others R. Shoeman, F. Stellato, C. H. Yoon, T. A. White, O. Yefanov, John Spence, H. N. Chapman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Current hard X-ray free-electron laser (XFEL) sources can deliver doses to biological macromolecules well exceeding 1 GGy, in timescales of a few tens of femtoseconds. During the pulse, photoionization can reach the point of saturation in which certain atomic species in the sample lose most of their electrons. This electronic radiation damage causes the atomic scattering factors to change, affecting, in particular, the heavy atoms, due to their higher photoabsorption cross sections. Here, it is shown that experimental serial femtosecond crystallography data collected with an extremely bright XFEL source exhibit a reduction of the effective scattering power of the sulfur atoms in a native protein. Quantitative methods are developed to retrieve information on the effective ionization of the damaged atomic species from experimental data, and the implications of utilizing new phasing methods which can take advantage of this localized radiation damage are discussed.

Original languageEnglish (US)
Article number041703
JournalStructural Dynamics
Volume2
Issue number4
DOIs
StatePublished - Jul 1 2015

Fingerprint

X ray lasers
Free electron lasers
Radiation damage
radiation damage
free electron lasers
Light sources
Laser pulses
damage
proteins
Proteins
Atoms
Photoionization
Crystals
Crystallography
Lattice vibrations
photoabsorption
pulses
Macromolecules
scattering
electronics

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Instrumentation
  • Radiation
  • Spectroscopy

Cite this

Galli, L., Son, S. K., Klinge, M., Bajt, S., Barty, A., Bean, R., ... Chapman, H. N. (2015). Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse. Structural Dynamics, 2(4), [041703]. https://doi.org/10.1063/1.4919398

Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse. / Galli, L.; Son, S. K.; Klinge, M.; Bajt, S.; Barty, A.; Bean, R.; Betzel, C.; Beyerlein, K. R.; Caleman, C.; Doak, R. B.; Duszenko, M.; Fleckenstein, H.; Gati, C.; Hunt, B.; Kirian, Richard; Liang, M.; Nanao, M. H.; Nass, K.; Oberthür, D.; Redecke, L.; Shoeman, R.; Stellato, F.; Yoon, C. H.; White, T. A.; Yefanov, O.; Spence, John; Chapman, H. N.

In: Structural Dynamics, Vol. 2, No. 4, 041703, 01.07.2015.

Research output: Contribution to journalArticle

Galli, L, Son, SK, Klinge, M, Bajt, S, Barty, A, Bean, R, Betzel, C, Beyerlein, KR, Caleman, C, Doak, RB, Duszenko, M, Fleckenstein, H, Gati, C, Hunt, B, Kirian, R, Liang, M, Nanao, MH, Nass, K, Oberthür, D, Redecke, L, Shoeman, R, Stellato, F, Yoon, CH, White, TA, Yefanov, O, Spence, J & Chapman, HN 2015, 'Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse', Structural Dynamics, vol. 2, no. 4, 041703. https://doi.org/10.1063/1.4919398
Galli, L. ; Son, S. K. ; Klinge, M. ; Bajt, S. ; Barty, A. ; Bean, R. ; Betzel, C. ; Beyerlein, K. R. ; Caleman, C. ; Doak, R. B. ; Duszenko, M. ; Fleckenstein, H. ; Gati, C. ; Hunt, B. ; Kirian, Richard ; Liang, M. ; Nanao, M. H. ; Nass, K. ; Oberthür, D. ; Redecke, L. ; Shoeman, R. ; Stellato, F. ; Yoon, C. H. ; White, T. A. ; Yefanov, O. ; Spence, John ; Chapman, H. N. / Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse. In: Structural Dynamics. 2015 ; Vol. 2, No. 4.
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AU - Bean, R.

AU - Betzel, C.

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AU - Nass, K.

AU - Oberthür, D.

AU - Redecke, L.

AU - Shoeman, R.

AU - Stellato, F.

AU - Yoon, C. H.

AU - White, T. A.

AU - Yefanov, O.

AU - Spence, John

AU - Chapman, H. N.

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