Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse

L. Galli, S. K. Son, M. Klinge, S. Bajt, A. Barty, R. Bean, C. Betzel, K. R. Beyerlein, C. Caleman, R. B. Doak, M. Duszenko, H. Fleckenstein, C. Gati, B. Hunt, Richard Kirian, M. Liang, M. H. Nanao, K. Nass, D. Oberthür, L. Redecke & 7 others R. Shoeman, F. Stellato, C. H. Yoon, T. A. White, O. Yefanov, John Spence, H. N. Chapman

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Current hard X-ray free-electron laser (XFEL) sources can deliver doses to biological macromolecules well exceeding 1 GGy, in timescales of a few tens of femtoseconds. During the pulse, photoionization can reach the point of saturation in which certain atomic species in the sample lose most of their electrons. This electronic radiation damage causes the atomic scattering factors to change, affecting, in particular, the heavy atoms, due to their higher photoabsorption cross sections. Here, it is shown that experimental serial femtosecond crystallography data collected with an extremely bright XFEL source exhibit a reduction of the effective scattering power of the sulfur atoms in a native protein. Quantitative methods are developed to retrieve information on the effective ionization of the damaged atomic species from experimental data, and the implications of utilizing new phasing methods which can take advantage of this localized radiation damage are discussed.

    Original languageEnglish (US)
    Article number041703
    JournalStructural Dynamics
    Volume2
    Issue number4
    DOIs
    StatePublished - Jul 1 2015

    Fingerprint

    X ray lasers
    Free electron lasers
    Radiation damage
    radiation damage
    free electron lasers
    Light sources
    Laser pulses
    damage
    proteins
    Proteins
    Atoms
    Photoionization
    Crystals
    Crystallography
    Lattice vibrations
    photoabsorption
    pulses
    Macromolecules
    scattering
    electronics

    ASJC Scopus subject areas

    • Condensed Matter Physics
    • Instrumentation
    • Radiation
    • Spectroscopy

    Cite this

    Galli, L., Son, S. K., Klinge, M., Bajt, S., Barty, A., Bean, R., ... Chapman, H. N. (2015). Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse. Structural Dynamics, 2(4), [041703]. https://doi.org/10.1063/1.4919398

    Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse. / Galli, L.; Son, S. K.; Klinge, M.; Bajt, S.; Barty, A.; Bean, R.; Betzel, C.; Beyerlein, K. R.; Caleman, C.; Doak, R. B.; Duszenko, M.; Fleckenstein, H.; Gati, C.; Hunt, B.; Kirian, Richard; Liang, M.; Nanao, M. H.; Nass, K.; Oberthür, D.; Redecke, L.; Shoeman, R.; Stellato, F.; Yoon, C. H.; White, T. A.; Yefanov, O.; Spence, John; Chapman, H. N.

    In: Structural Dynamics, Vol. 2, No. 4, 041703, 01.07.2015.

    Research output: Contribution to journalArticle

    Galli, L, Son, SK, Klinge, M, Bajt, S, Barty, A, Bean, R, Betzel, C, Beyerlein, KR, Caleman, C, Doak, RB, Duszenko, M, Fleckenstein, H, Gati, C, Hunt, B, Kirian, R, Liang, M, Nanao, MH, Nass, K, Oberthür, D, Redecke, L, Shoeman, R, Stellato, F, Yoon, CH, White, TA, Yefanov, O, Spence, J & Chapman, HN 2015, 'Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse' Structural Dynamics, vol. 2, no. 4, 041703. https://doi.org/10.1063/1.4919398
    Galli, L. ; Son, S. K. ; Klinge, M. ; Bajt, S. ; Barty, A. ; Bean, R. ; Betzel, C. ; Beyerlein, K. R. ; Caleman, C. ; Doak, R. B. ; Duszenko, M. ; Fleckenstein, H. ; Gati, C. ; Hunt, B. ; Kirian, Richard ; Liang, M. ; Nanao, M. H. ; Nass, K. ; Oberthür, D. ; Redecke, L. ; Shoeman, R. ; Stellato, F. ; Yoon, C. H. ; White, T. A. ; Yefanov, O. ; Spence, John ; Chapman, H. N. / Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse. In: Structural Dynamics. 2015 ; Vol. 2, No. 4.
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    AU - Barty, A.

    AU - Bean, R.

    AU - Betzel, C.

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    AU - Doak, R. B.

    AU - Duszenko, M.

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    AU - Gati, C.

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    AU - Kirian, Richard

    AU - Liang, M.

    AU - Nanao, M. H.

    AU - Nass, K.

    AU - Oberthür, D.

    AU - Redecke, L.

    AU - Shoeman, R.

    AU - Stellato, F.

    AU - Yoon, C. H.

    AU - White, T. A.

    AU - Yefanov, O.

    AU - Spence, John

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