Electron spectroscopy and atomic force microscopy studies of DNA adsorption on mica

C. E. Rabke, L. A. Wenzler, T. P. Beebe, S. M. Lindsay, R. Wepf, T. Thundat, R. L. McCarley, J. Vesenka, O. Johari

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Various methods for the deposition of deoxyribonucleic acid (DNA) molecules on mica are investigated to determine their reproducibility, and to quantify their ability to bind DNA. The use of these deposition methods for sample preparation for biological scanning tunneling microscopy (STM) and atomic force microscopy (AFM) studies is discussed. Auger electron spectroscopy (AES) and electron spectroscopy for chemical analysis (ESCA) were used to investigate the quantity of DNA adsorbed. AFM images of DNA deposited using the methods investigated are presented. The combination of AFM results with AES and ESCA results provides a basic understanding of the deposition techniques studied and illustrates that electron spectroscopy can be a useful addition to studies of this nature.

Original languageEnglish (US)
Pages (from-to)471-480
Number of pages10
JournalScanning Microscopy
Volume8
Issue number3
StatePublished - 1994
Externally publishedYes

Keywords

  • Auger electron spectroscopy
  • Br-DNA
  • Mg Acetate
  • Sulfur-modified-DNA
  • atomic force microscopy
  • electron spectroscopy for chemical analysis
  • mica
  • scanning tunneling microscopy

ASJC Scopus subject areas

  • Instrumentation

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