Electron microscopic study of nuclear and nucleolar changes in cells infected with canine distemper virus (CDV)

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The nuclear changes occurring in dog kidney cells infected with a virulent and an attenuated strain of canine distemper virus were examined by electron microscopy. No ultrastructural evidence for intranuclear virus replication was observed even though large eosinophilic nuclear inclusions were found in up to 34% of infected cells examined by light microscopy. This discrepancy between light and electron microscopic findings was discussed in relation to the possible structure of the nuclear inclusions. Marked morphological changes were noted in the nucleoli of up to 12% of cells infected with the virulent strain, CDV/BR, involving complete segregation of the granular and fibrillar components. The nucleolar lesions were similar to those induced by chemical inhibitors of cellular nucleic acid metabolism.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalArchiv für die gesamte Virusforschung
Volume37
Issue number2-3
DOIs
StatePublished - Jun 1972
Externally publishedYes

Fingerprint

Canine Distemper Virus
Intranuclear Inclusion Bodies
Electrons
Light
Virus Replication
Nucleic Acids
Microscopy
Electron Microscopy
Dogs
Kidney

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics
  • Medicine(all)

Cite this

@article{576f0bc4766d4754b94be576c075cbb2,
title = "Electron microscopic study of nuclear and nucleolar changes in cells infected with canine distemper virus (CDV)",
abstract = "The nuclear changes occurring in dog kidney cells infected with a virulent and an attenuated strain of canine distemper virus were examined by electron microscopy. No ultrastructural evidence for intranuclear virus replication was observed even though large eosinophilic nuclear inclusions were found in up to 34{\%} of infected cells examined by light microscopy. This discrepancy between light and electron microscopic findings was discussed in relation to the possible structure of the nuclear inclusions. Marked morphological changes were noted in the nucleoli of up to 12{\%} of cells infected with the virulent strain, CDV/BR, involving complete segregation of the granular and fibrillar components. The nucleolar lesions were similar to those induced by chemical inhibitors of cellular nucleic acid metabolism.",
author = "George Poste",
year = "1972",
month = "6",
doi = "10.1007/BF01268000",
language = "English (US)",
volume = "37",
pages = "183--190",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "2-3",

}

TY - JOUR

T1 - Electron microscopic study of nuclear and nucleolar changes in cells infected with canine distemper virus (CDV)

AU - Poste, George

PY - 1972/6

Y1 - 1972/6

N2 - The nuclear changes occurring in dog kidney cells infected with a virulent and an attenuated strain of canine distemper virus were examined by electron microscopy. No ultrastructural evidence for intranuclear virus replication was observed even though large eosinophilic nuclear inclusions were found in up to 34% of infected cells examined by light microscopy. This discrepancy between light and electron microscopic findings was discussed in relation to the possible structure of the nuclear inclusions. Marked morphological changes were noted in the nucleoli of up to 12% of cells infected with the virulent strain, CDV/BR, involving complete segregation of the granular and fibrillar components. The nucleolar lesions were similar to those induced by chemical inhibitors of cellular nucleic acid metabolism.

AB - The nuclear changes occurring in dog kidney cells infected with a virulent and an attenuated strain of canine distemper virus were examined by electron microscopy. No ultrastructural evidence for intranuclear virus replication was observed even though large eosinophilic nuclear inclusions were found in up to 34% of infected cells examined by light microscopy. This discrepancy between light and electron microscopic findings was discussed in relation to the possible structure of the nuclear inclusions. Marked morphological changes were noted in the nucleoli of up to 12% of cells infected with the virulent strain, CDV/BR, involving complete segregation of the granular and fibrillar components. The nucleolar lesions were similar to those induced by chemical inhibitors of cellular nucleic acid metabolism.

UR - http://www.scopus.com/inward/record.url?scp=0015252935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015252935&partnerID=8YFLogxK

U2 - 10.1007/BF01268000

DO - 10.1007/BF01268000

M3 - Article

C2 - 4112842

AN - SCOPUS:0015252935

VL - 37

SP - 183

EP - 190

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 2-3

ER -